Enzymatic determination of estradiol and estrone in plasma and urine
| Autor: | Anne-Marie Boussioux, AndréCrastes de Paulet, Jean-Claude Nicolas, Bernard Descomps |
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| Rok vydání: | 1979 |
| Předmět: |
Estrone
medicine.drug_class Clinical Biochemistry Estradiol Dehydrogenases Urine Biochemistry chemistry.chemical_compound Pregnancy Spectrophotometry Methods medicine Humans chemistry.chemical_classification Chromatography Estradiol medicine.diagnostic_test Biochemistry (medical) Extraction (chemistry) Human placenta General Medicine Menstruation Enzyme chemistry Evaluation Studies as Topic Estrogen Female hormones hormone substitutes and hormone antagonists |
| Zdroj: | Clinica Chimica Acta. 92:1-9 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(79)90389-9 |
| Popis: | Plasma and urine estradiol and estrone can be determined using the transhydrogenase function of the estradiol dehydrogenase of human placenta: in the conditions described the transhydrogenase activity which is directly related to the estrogen concentration is measured by spectrophotometry. Estrone and estradiol can be determined together or separately if estrone is previously reacted with hydrazine with a limit of sensitivity of 10 picograms in the sample. On urine samples the assay is performed directly without extraction, a simplification which makes the method highly suitable for numerous routine determinations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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