High-fat diet and caffeine interact to modulate bone microstructure and biomechanics in mice

Autor: Felipe Couto Santos, Ana Cláudia Ferreira Souza, Fernanda Batista de Souza, Daniel Silva Sena Bastos, Reggiani Vilela Gonçalves, Eliziária C. Santos, Cynthia Fernandes Ferreira Santos, Luiz Carlos Maia Ladeira, Mariana Machado-Neves, Franciele Angelo de Deus, Rômulo Dias Novaes
Rok vydání: 2021
Předmět:
0301 basic medicine
medicine.medical_specialty
chemistry.chemical_element
Cafeteria
Calcium
Diet
High-Fat
030226 pharmacology & pharmacy
General Biochemistry
Genetics and Molecular Biology
Bone and Bones
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Internal medicine
Caffeine
medicine
Animals
Tibia
General Pharmacology
Toxicology and Pharmaceutics
medicine.diagnostic_test
biology
Cholesterol
business.industry
digestive
oral
and skin physiology
Body Weight
food and beverages
nutritional and metabolic diseases
General Medicine
biology.organism_classification
Biomechanical Phenomena
Mice
Inbred C57BL
030104 developmental biology
Endocrinology
medicine.anatomical_structure
chemistry
lipids (amino acids
peptides
and proteins)
Central Nervous System Stimulants
Female
medicine.symptom
Lipid profile
business
Cancellous bone
Weight gain
Zdroj: Life sciences. 276
ISSN: 1879-0631
Popis: Aims Although excessive fat and caffeine intake are independent risk factors for bone microstructural and functional disturbances, their association remains overlooked. Thus, we investigated the impact of high-fat diet (HFD) and caffeine alone and combined on serum lipid profile, bone microstructure, micromineral distribution and biomechanical properties. Methods Forty female C57BL/6 mice were randomized into 4 groups daily treated for seventeen weeks with standard diet (SD) or HFD (cafeteria diet) alone or combined with 50 mg/kg caffeine. Key findings The association between HFD and caffeine reduced the weight gain compared to animals receiving HFD alone. Caffeine alone or combined with HFD increases total and HDL cholesterol circulating levels. HFD also reduced calcium, phosphorus and magnesium bone levels compared to the groups receiving SD, and this reduction was aggravated by caffeine coadministration. From biomechanical assays, HFD combined with caffeine increased bending strength and stiffness of tibia, a finding aligned with the marked microstructural remodeling of the cortical and cancellous bone in animals receiving this combination. Significance Our findings indicated that HFD and caffeine interact to induce metabolic changes and bone microstructural remodeling, which are potentially related to bone biomechanical adaptations in response to HFD and caffeine coadministration.
Databáze: OpenAIRE
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