High performance liquid chromatographic analysis of cholesterol in milk
| Autor: | W. Jeffrey Hurst, M. D. Aleo, Robert A. Martin |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Potassium hydroxide
Chromatography Silica gel Extraction (chemistry) Ether High-performance liquid chromatography Hexane chemistry.chemical_compound Cholesterol Milk chemistry Genetics Animals Animal Science and Zoology Sample preparation Cattle Saponification Chromatography High Pressure Liquid Food Science |
| Zdroj: | Journal of dairy science. 66(10) |
| ISSN: | 0022-0302 |
| Popis: | A method was developed for analysis of cholesterol in whole milk powder and commercial liquid milk products. High performance liquid chromatography employed a nonaqueous reversed phase chromatographic system. Sample preparation was by saponification with methanolic potassium hydroxide followed by extraction with ether. Potential interfering substances were removed prior to injection by a simple clean-up procedure using a silica gel Sep-pak TM , The chromatographic system included a carbon-18 column with a hexane/isopropanol mobile phase and detection at 205nm. Method validation and recovery studies using carbon–14 labeled cholesterol indicated excellent performance. |
| Databáze: | OpenAIRE |
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