Heteroduplex mobility assay for the identification ofListeriasp. andListeria monocytogenesstrains: application to characterisation of strains from sludge and food samples
| Autor: | N. Garrec, A.M Pourcher, M. Marault, A. Kerouanton, A. Brisabois, Laurent Sutra |
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| Rok vydání: | 2003 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Serotype Listeria Bacterial Toxins Immunology Sewage Heteroduplex Analysis Biology medicine.disease_cause DNA Ribosomal Polymerase Chain Reaction Microbiology Hemolysin Proteins Listeria monocytogenes RNA Ribosomal 16S Genotype medicine Animals Humans Immunology and Allergy Serotyping Heat-Shock Proteins business.industry Genes rRNA General Medicine 16S ribosomal RNA biology.organism_classification Bacterial Typing Techniques Infectious Diseases Food Microbiology Carbohydrate Metabolism business Bacteria Sludge |
| Zdroj: | FEMS Immunology & Medical Microbiology. 38:257-264 |
| ISSN: | 1574-695X 0928-8244 |
| Popis: | One hundred and ten Listeria sp. isolates from sewage sludge were identified according to phenotypic and genotypic methods. The Listeria sp. strains isolated from five types of sludge from three sewage treatment plants in Angers (France) and the surrounding area included L. monocytogenes (55.5%), L. innocua (29.1%), L. seeligeri (13.6%) and L. welshimeri (1.8%). The majority of L. monocytogenes strains belonged to serotypes 4b, 1/2b and 1/2a. Moreover, a heteroduplex mobility assay based on the 16S rRNA sequences was tested for its ability to identify the six species of the genus Listeria. This study, performed on 283 Listeria sp. strains from human, food and sewage sludge samples, showed that all the species were distinguishable from one another. L. innocua and L. seeligeri showed respectively three and two distinct banding patterns. Within L. monocytogenes, four groups (I-IV) were defined. The majority of food and environmental isolates were clustered in group I and it is noteworthy that group IV clustered epidemiologic isolates and strains belonging to serotypes 4b, 1/2a and 1/2b. |
| Databáze: | OpenAIRE |
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