Multiple drug resistance of Campylobacter jejuni and Shigella isolated from diarrhoeic children at Kapsabet County referral hospital, Kenya
Autor: | Mwamburi Lizzy, Mutuku M. Angela, Kakai Rose, Ongwae H. Zachariah |
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Rok vydání: | 2021 |
Předmět: |
Diarrhea
Male 0301 basic medicine Veterinary medicine Antibiotic resistance 030106 microbiology Erythromycin Microbial Sensitivity Tests medicine.disease_cause Campylobacter jejuni lcsh:Infectious and parasitic diseases Feces 03 medical and health sciences 0302 clinical medicine Drug Resistance Multiple Bacterial Ampicillin Campylobacter Infections medicine Humans lcsh:RC109-216 Shigella 030212 general & internal medicine Child Dysentery Bacillary biology business.industry Sulfamethoxazole Infant biology.organism_classification Kenya Trimethoprim Hospitals Diarrhoea Anti-Bacterial Agents Multiple drug resistance Infectious Diseases Child Preschool Female business Research Article medicine.drug |
Zdroj: | BMC Infectious Diseases, Vol 21, Iss 1, Pp 1-8 (2021) BMC Infectious Diseases |
ISSN: | 1471-2334 |
Popis: | Background Diarrhoea is a common cause of mortality and morbidity in children under five years old. In Kenya, it has a 21% case fatality with Enteropathogenic E. coli, Campylobacter jejuni, Shigella spp. and Salmonella spp. accounting for 50–60% of the cases. Sulphonamides, tetracycline, ampicillin and trimethoprim/sulfamethoxazole are typically used in the treatment of diarrhoeal diseases but have become ineffective in the face of emerging antimicrobial resistance. The objective of this study was to evaluate the prevalence and antimicrobial susceptibility of Campylobacter jejuni and Shigella species in children under five years of age presenting with diarrhoea at Kapsabet County Referral Hospital in Kenya. Methods Faecal samples were collected from 139 children admitted with diarrhoea. Each sample was examined macroscopically for colour, texture, and presence of extraneous material. The samples were then cultured for bacterial growth. Observed bacterial growth was isolated and identified by a series of biochemical tests. Resistance patterns were also evaluated using the Kirby – Bauer Disk diffusion method. The chi – square test and Pearson Correlation Coefficient were used to establish statistical significance. Results Approximately 33.1% of the total faecal samples tested were positive for enteric pathogens. Shigella spp. demonstrated resistance to erythromycin (91.7%), doxycyclin (83.3%), ampicillin (82.1%), cotrimoxazole (73.1%), minocycline (66.7%) and cefuroxime (54.2%). Campylobacter jejuni also exhibited resistance to erythromycin (87.5%), doxycyclin (75%), ampicillin (73.7%), cotrimoxazole (73.3%) and minocycline (68.8%). Conclusions The resistance patterns of Shigella spp. and Campylobacter jejuni reported in this study necessitates the need for a comprehensive multiregional investigation to evaluate the geographical prevalence and antimicrobial resistance distributions of these microorganisms. These findings also support the need for the discovery and development of effective therapeutic alternatives. Trial registration Retrospectively registered. Certificate No. 00762 |
Databáze: | OpenAIRE |
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