| Popis: |
To compare two commercial formulations of alfaxalone for immersion anaesthesia in laboratory zebrafish.Prospective, blinded, randomized study.A total of 20 adult Danio rerio (Tuebingen strain).Zebrafish were divided into two groups of 10 (five female, five male) and placed in individual immersion baths containing 10 mg LThere were no statistical differences between groups in reported variables. TPT, AIT and ART were 10.2 ± 1.2, 1.9 ± 0.9 and 8.3 ± 1.2 minutes for group 1 and 10.8 ± 2.9, 2.4 ± 1.2 and 8.4 ± 2.7 minutes for group 2. No gross external pathology was evident, and no fish died during the experimental period. Histopathology showed normal gill pathology and no difference between the groups.Immersion anaesthesia using 10 mg L |
