Cardiac arrhythmias after renal I/R depend on IL-1β

Autor: Pedrosa Roberto Coury, Wellington Caio-Silva, Karine Panico, Ygor Schleier, Thabata Duque, Fabianno Ferreira, Ainhoa Rodriguez de Yurre, Oscar Moreno-Loaiza, Marcela Sorelli Carneiro-Ramos, Emiliano Medei, Claudia N. Paiva, Maria Micaela Lopez Alarcon, Mayra Trentin-Sonoda
Rok vydání: 2019
Předmět:
Zdroj: Journal of molecular and cellular cardiology. 131
ISSN: 1095-8584
Popis: Aims Cardiac arrhythmias are one of the most important remote complications after kidney injury. Renal ischemia reperfusion (I/R) is a major cause of acute renal injury predisposing to several remote dysfunctions, including cardiac electrical disturbance. Since IL-1β production dependent on NLRP3 represents a link between tissue malfunctioning and cardiac arrhythmias, here we tested the hypothesis that longer ventricular repolarization and arrhythmias after renal I/R depend on this innate immunity sensor. Methods and results Nlrp3−/− and Casp1−/− mice reacted to renal I/R with no increase in plasma IL-1β, different from WT (wild-type) I/R. A prolonged QJ interval and an increased susceptibility to ventricular arrhythmias were found after I/R compared to Sham controls in wild-type mice at 15 days post-perfusion, but not in Nlrp3−/− or CASP1−/− I/R, indicating that the absence of NLRP3 or CASP1 totally prevented longer QJ interval after renal I/R. In contrast with WT mice, we found no renal atrophy and no renal dysfunction in Nlrp3−/− and Casp1−/− mice after renal I/R. Depletion of macrophages in vivo after I/R and a day before IL-1β peak (at 7 days post-perfusion) totally prevented prolongation of QJ interval, suggesting that macrophages might participate as sensors of tissue injury. Moreover, treatment of I/R-WT mice with IL-1r antagonist (IL-1ra) from 8 to 15 days post perfusion did not interfere with renal function, but reversed QJ prolongation, prevented the increase in susceptibility to ventricular arrhythmias and rescued a close to normal duration and amplitude of calcium transient. Conclusion Taken together, these results corroborate the hypothesis that IL-1β is produced after sensing renal injury through NRLP3-CASP1, and IL-1β on its turn triggers longer ventricular repolarization and increase susceptibility to cardiac arrhythmias. Still, they offer a therapeutic approach to treat cardiac arrhythmias that arise after renal I/R.
Databáze: OpenAIRE