17β-Estradiol protects depletion of rat temporal cortex somatostatinergic system by β-amyloid
| Autor: | Jesús Argente, David Aguado-Llera, Laura M. Frago, L. Puebla-Jiménez, Julie A. Chowen, Eduardo Arilla-Ferreiro, Vicente Barrios |
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| Rok vydání: | 2007 |
| Předmět: |
Aging
medicine.medical_specialty Ovariectomy Radioimmunoassay Estrogen receptor Biology Neuroprotection Adenylyl cyclase chemistry.chemical_compound Internal medicine In Situ Nick-End Labeling medicine Animals RNA Messenger Receptors Somatostatin Rats Wistar Receptor Fulvestrant Protein kinase B Temporal cortex Analysis of Variance Amyloid beta-Peptides Cell Death Estradiol Reverse Transcriptase Polymerase Chain Reaction Brain-Derived Neurotrophic Factor General Neuroscience Estrogen Antagonists Peptide Fragments Temporal Lobe Rats Endocrinology Somatostatin chemistry Ovariectomized rat Female Neurology (clinical) Geriatrics and Gerontology hormones hormone substitutes and hormone antagonists Adenylyl Cyclases Protein Binding Signal Transduction Developmental Biology |
| Zdroj: | Neurobiology of Aging. 28:1396-1409 |
| ISSN: | 0197-4580 |
| Popis: | Estradiol prevents amyloid-beta peptide (Abeta)-induced cell death through estrogen receptors (ERs) and modulates somatostatin (SRIF) responsiveness in the rat brain. As intracerebroventricular (ICV) Abeta25-35 administration reduces SRIFergic tone in the temporal cortex of ovariectomized (Ovx) rats, we asked whether 17beta-estradiol (E2) treatment can restore the Abeta25-35 induced changes in SRIF content, SRIF receptor density and adenylyl cyclase (AC) activity, as well as if these effects are mediated by ERs. E2 treatment did not change Abeta25-35 levels in the temporal cortex, but partially restored the SRIFergic parameters affected by Abeta insult and decreased cell death, which was correlated with Akt activation. The ER antagonist ICI 182,780 prevented the protective effect of E2 on sst2 levels, but did not modify SRIF levels. Furthermore, ICI 182,780 treatment further decreased sst2 protein and mRNA levels when administered alone to Abeta25-35-treated rats, suggesting that it may block the effects of endogenous estrogens. These findings indicate that E2 protects the temporal cortical SRIFergic system from Abeta-induced depletion independently of Abeta accumulation. |
| Databáze: | OpenAIRE |
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