Pleiotropic Functions of High Fat Diet in the Etiology of Osteoarthritis
| Autor: | Maierhaba Ailixiding, Yasushi Hara, Yoshinori Asou, Munetaka Iwata, Zulipiya Aibibula, Guangwen Jin, Atsushi Okawa, Hiroki Ochi, Jinying Piao |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cartilage Articular Pathology lcsh:Medicine Apoptosis Osteoarthritis Biochemistry Weight-Bearing Fats Mice 0302 clinical medicine Animal Cells Medicine and Health Sciences lcsh:Science Connective Tissue Cells Staining Multidisciplinary TUNEL assay Infrapatellar fat pad Cell Death digestive oral and skin physiology Osteophyte food and beverages Organ Size Animal Models Osteoarthritis Knee Lipids Biomechanical Phenomena medicine.anatomical_structure Adipose Tissue Lower Extremity Connective Tissue Cell Processes lipids (amino acids peptides and proteins) medicine.symptom Anatomy Cellular Types hormones hormone substitutes and hormone antagonists Research Article medicine.medical_specialty Normal diet Adipokine Inflammation Mouse Models Diet High-Fat Real-Time Polymerase Chain Reaction Research and Analysis Methods Chondrocyte Proinflammatory cytokine 03 medical and health sciences Chondrocytes Model Organisms Rheumatology Internal medicine medicine In Situ Nick-End Labeling Animals RNA Messenger Cytoplasmic Staining Nutrition 030203 arthritis & rheumatology business.industry Arthritis lcsh:R Body Weight nutritional and metabolic diseases Biology and Life Sciences X-Ray Microtomography Cell Biology medicine.disease Diet Mice Inbred C57BL 030104 developmental biology Endocrinology Biological Tissue Cartilage Specimen Preparation and Treatment lcsh:Q Safranin Staining business Articular Cartilage |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 9, p e0162794 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Obesity is a risk factor for osteoarthritis (OA). To investigate the roles of increased mechanical loading in the onset of obesity-induced OA, knee joints were histologically analyzed after applying a tail suspension (TS) model to a high-fat diet (HFD)-induced OA model. Mice were divided into four groups: normal diet (ND) with normal loading (NL) group; HFD with NL group; ND with TS group; and HFD with TS group. Whole knee joints were evaluated by immunohistological analysis. The infrapatellar fat pad (IPFP) was excised and mRNA expression profiles were compared by qPCR analysis. After twelve weeks of the diet, body weight was increased by HFD in both the NL group and TS group. Upon histological analysis, the irregularity of the surface layer of articular cartilage was observed only in the NL+HFD group. Osteophyte area increased as a result of HFD in both the NL and TS groups, although osteophyte area in the TS+HFD group was smaller than that of the NL+HFD group. In the evaluation of the IPFP by qPCR, adipokines and inflammatory cytokines also increased as a result of HFD. While TGF-β increased as a result of HFD, the trend was slightly lower in the TS group, in parallel with osteophyte area. To detect apoptosis of articular chondrocytes, TUNEL staining was employed. TUNEL-positive cells were abundantly observed in the articular cartilage in the HFD mice regardless of mechanical loading. IPFP inflammation, enhanced chondrocyte apoptosis, and osteophyte formation were seen even in the TS group as a result of a HFD. In all, these data demonstrate that HFD contributed to osteophyte formation through mechanical loading dependent and independent mechanisms. |
| Databáze: | OpenAIRE |
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