Metabotropic glutamate receptors in cultured cerebellar granule cells: developmental profile

Autor: Eleonora Aronica, P. Dell'Albani, Carla Amico, R. Balázs, F. Nicoletti, Daniele F. Condorelli
Přispěvatelé: Other departments
Jazyk: angličtina
Rok vydání: 1993
Předmět:
Cerebellum
Time Factors
Messenger
genetics/metabolism
Phosphatidylinositols
Biochemistry
Glutamates
Phosphatidylinositol Phosphates
Analysis of Variance
Animals
Blotting
Northern
Cells
Cultured
Cerebellum
cytology/metabolism
Dizocilpine Maleate
pharmacology
Glutamates
pharmacology
Glutamic Acid
Inositol Phosphates
metabolism
Inositol
metabolism
Kinetics
Neurons
cytology/drug effects/metabolism
Phosphatidylinositol Phosphates
Phosphatidylinositols
metabolism
RNA
isolation /&/ purification/metabolism
RNA
isolation /&/ purification/metabolism
Rats
Receptors
Glutamate
genetics/metabolism
Receptors
N-Methyl-D-Aspartate
antagonists /&/ inhibitors
Time Factors
Receptors
Northern
Receptor
Cells
Cultured
cytology/metabolism
antagonists /&/ inhibitors
Neurons
Cultured
Blotting
Glutamate receptor
Receptor antagonist
medicine.anatomical_structure
Receptors
Glutamate
NMDA receptor
Metabotropic glutamate receptor 1
medicine.drug_class
Cells
Inositol Phosphates
Glutamic Acid
Biology
Receptors
N-Methyl-D-Aspartate
Cellular and Molecular Neuroscience
medicine
Animals
RNA
Messenger
Analysis of Variance
isolation /&/ purification/metabolism
cytology/drug effects/metabolism
Blotting
Northern
Molecular biology
Rats
Kinetics
Metabotropic receptor
Metabotropic glutamate receptor
RNA
pharmacology
Dizocilpine Maleate
metabolism
Inositol
Zdroj: Journal of neurochemistry, 60(2), 559-565. Wiley-Blackwell
Scopus-Elsevier
ISSN: 0022-3042
Popis: Excitatory amino acid (EAA)-induced polyphosphoinositide (PPI) hydrolysis was studied during the development in culture of cerebellar granule cells. The developmental pattern was similar using metabotropic glutamate (Glu) receptor (mGluR) agonists, including L-Glu, quisqualate, and trans-(+/-)-1-amino-1,3-cyclopentanedicarboxylic acid: The stimulation of [3H]inositol monophosphate ([3H]-InsP) formation was low at 2 days in vitro (DIV), but the response increased steeply, reaching a peak at 4 DIV, followed by a progressive decline. In contrast, carbamylcholine-induced PPI hydrolysis exhibited a plateau after a pronounced increase during the first week in vitro. At 6 DIV, but not at 4 DIV, when the activity peaked, PPI hydrolysis elicited by Glu was reduced by the N-methyl-D-aspartate (NMDA) receptor antagonist MK-801, indicating that in cultured granule cells, NMDA receptors contribute to [3H]-InsP formation and that this component of the response develops relatively late. Accordingly, NMDA-induced [3H]-InsP formation, estimated under Mg(2+)-free conditions, increased markedly from very low values at 2 DIV to a plateau at 8-10 DIV. The developmental pattern of EAA-induced PPI hydrolysis was paralleled by changes in the level of an mRNA for a specific mGluR subtype (mGluR1 mRNA). RNA blot analysis performed with the pmGR1 cDNA probe revealed that the hybridization signal in RNA extracts from cultures at 1 DIV was very weak, but mGluR mRNA levels increased dramatically between 1 and 3 DIV, followed by a progressive decrease, so that by 15 DIV the mRNA levels were only approximately 10% of the values at 3 DIV.(ABSTRACT TRUNCATED AT 250 WORDS)
Databáze: OpenAIRE
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