Human Cordonal Stem Cell Intraperitoneal Injection Can Represent a Rescue Therapy After an Acute Hepatic Damage in Immunocompetent Rats
| Autor: | Giovanni Gasbarrini, Fabio Maria Vecchio, S. Mancuso, Sergio Rutella, Giovanni Monego, Anna Chiara Piscaglia, C. Di Campli, G. Di Gioacchino, Giuseppe Leone, Antonio Gasbarrini, Maria Assunta Zocco, G. Bonanno, Fabrizio Michetti, Paolo Pola, Marialuisa Novi |
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| Rok vydání: | 2005 |
| Předmět: |
Pathology
medicine.medical_specialty Kupffer Cells Propanols Settore MED/12 - GASTROENTEROLOGIA medicine.medical_treatment Transplantation Heterologous Intraperitoneal injection Human cordonal stem cell medicine Animals Humans Intraperitoneal Rats Wistar human cordonal Settore BIO/16 - ANATOMIA UMANA Transplantation business.industry Liver Diseases Hematopoietic stem cell Flow Cytometry Immunohistochemistry Liver regeneration Rats stem cell Disease Models Animal Haematopoiesis medicine.anatomical_structure Cord blood Hepatic stellate cell Surgery Cord Blood Stem Cell Transplantation Chemical and Drug Induced Liver Injury Stem cell business Adult stem cell |
| Zdroj: | Transplantation Proceedings. 37:2711-2714 |
| ISSN: | 0041-1345 |
| DOI: | 10.1016/j.transproceed.2005.06.076 |
| Popis: | Background and Aim Tissue homeostasis and turnover require reserve stem proliferating cells. Several studies performed on immunodeficient animals have suggested a degree of plasticity by the hematopoietic stem cell compartment that may represent source for liver regeneration. We sought to explore the hepatic differentiation potential of hematopoietic stem cells from human cord blood, after toxic liver damage induced by allyl-alcohol in immunocompetent rats. Materials and Methods Wistar rats were divided into groups (A) allyl-alcohol intraperitoneal injection with hematopoietic stem cell intraperitoneal infusion at 1 day and sacrifice 3 days later; (B) stem cell injection and sacrifice 3 days later; (C) allyl-alcohol infusion and sacrifice 4 days later; and (D) sacrifice without any treatment. Livers, spleens, and bone marrows were analysed for human stem cells using flow-cytometry; livers were also tested by histology and immunohistochemistry to study the pattern of hepatic regeneration after damage and human stem cell conversion into hepatocyte-like cells, respectively. Results Flow-cytometry revealed selective recruitment of human hematopoietic stem cells by damaged livers (group A) compared with control group B. In addition, liver damage was reduced in animals treated with stem cells. Immunohistochemistry demonstrated that human stem cells could convert hepatic cells. Conclusions Our study demonstrated that hematopoietic stem cells selectively recruited by injured livers can contribute to hepatic regeneration after acute toxic damage in immunocompetent recipients. |
| Databáze: | OpenAIRE |
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