Interaction Domains and Nuclear Targeting Signals in Subunits of the U2 Small Nuclear Ribonucleoprotein Particle-associated Splicing Factor SF3a
| Autor: | Angela Krämer, Fabio Ferfoglia, Flore Raleff, Ching-Jung Huang |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Cytoplasm
Cell Nucleus/genetics/metabolism Amino Acid Motifs Nuclear Localization Signals Active Transport Cell Nucleus Nuclear Localization Signals/genetics/metabolism Biology Heterogeneous ribonucleoprotein particle Biochemistry 03 medical and health sciences 0302 clinical medicine ddc:570 Cytoplasm/genetics/metabolism Humans snRNP Active Transport Cell Nucleus/physiology Nuclear export signal Molecular Biology 030304 developmental biology Ribonucleoprotein Cell Nucleus 0303 health sciences Ribonucleoprotein U2 Small Nuclear/genetics/metabolism Nuclear cap-binding protein complex Small Nuclear Ribonucleoprotein Particle Cell Biology Ribonucleoprotein U2 Small Nuclear Molecular biology Cell biology Protein Structure Tertiary Hela Cells RNA splicing Nuclear lamina RNA 030217 neurology & neurosurgery HeLa Cells |
| Zdroj: | JOURNAL OF BIOLOGICAL CHEMISTRY Journal of Biological Chemistry, Vol. 286, No 15 (2011) pp. 13106-14 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.M110.201491 |
| Popis: | Human splicing factor SF3a is a component of the mature U2 small nuclear ribonucleoprotein particle (snRNP) and its three subunits of 60 66 and 120 kDa are essential for splicing in vitro and in vivo. The SF3a heterotrimer forms in the cytoplasm and enters the nucleus independently of the U2 snRNP. Here we have analyzed domains required for in vitro interactions between the SF3a subunits. Our results indicate that the SF3a66 SF3a120 interaction is mediated by a 27 amino acid region in SF3a120 C terminal to the second suppressor of white apricot and prp21/spp91 domain and amino acids 108 210 of SF3a66. Neither of these sequences contains known structural motifs suggesting that the interaction domains are novel. Moreover an similar to 100 amino acid region including the SURP2 domain of SF3a120 but extending into neighboring regions is sufficient for binding to SF3a60. Analysis of determinants for nuclear import of SF3a demonstrates that SF3a120 provides the major nuclear localization signal and SF3a60 contributes to nuclear import. |
| Databáze: | OpenAIRE |
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