A putative integrase gene defines the distal end of a large cluster of ToxR-regulated colonization genes in Vibrio cholerae
| Autor: | Michael E. Kovach, Megan D. Shaffer, Kenneth M. Peterson |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
DNA
Bacterial Genotype Transcription Genetic Molecular Sequence Data Restriction Mapping Asiatic cholera medicine.disease_cause Regulon Microbiology Bacterial Proteins event.disaster Gene cluster Escherichia coli medicine Recombinase event Amino Acid Sequence Vibrio cholerae Gene Prophage Genetics Base Sequence Integrases Sequence Homology Amino Acid Virulence biology Gene Expression Regulation Bacterial biology.organism_classification Vibrio Integrase DNA-Binding Proteins RNA Bacterial Multigene Family DNA Nucleotidyltransferases biology.protein Plasmids Transcription Factors |
| Zdroj: | Microbiology. 142:2165-2174 |
| ISSN: | 1465-2080 1350-0872 |
| Popis: | A large cluster of virulence genes encoding proteins involved in Vibrio cholerae accessory colonization factor (ACF) expression and toxin-coregulated pilus (TCP) biogenesis is flanked by sequences that resemble bacteriophage attachment (att) half-sites. Adjacent to the attL-like site is a gene (int) that encodes a protein related to the integrase family of site-specific recombinases. The putative vibrio integrase appears to be most closely related to the Escherichia coli cryptic prophage (CP4-57) integrase protein (52% identity, 73% similarity). Genomic analysis of numerous V. cholerae strains (O1, non-O1 and O139) revealed that only vibrios capable of causing epidemic Asiatic cholera possess the TCP-ACF colonization gene cluster in association with the integrase. The fact that the integrase gene is absent in avirulent strains suggests that epidemic strains of V. cholerae obtained the TCP-ACF colonization gene cluster via horizontal transfer. |
| Databáze: | OpenAIRE |
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