Enhanced protein levels of protein thiol/disulphide oxidoreductases in placentae from pre-eclamptic subjects
| Autor: | Hiroki Nanri, K. Ejima, C. Koyama, N. Toki, E. Shibata, Masaharu Ikeda, Masamichi Kashimura |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Antioxidant medicine.medical_treatment Placenta Immunoblotting Protein Disulfide-Isomerases Gestational Age Biology medicine.disease_cause Lipid peroxidation chemistry.chemical_compound Thioredoxins Pre-Eclampsia Pregnancy Glutaredoxin medicine Humans reproductive and urinary physiology Glutaredoxins chemistry.chemical_classification Reactive oxygen species Aldehydes Obstetrics and Gynecology Proteins Protein Disulfide Reductase (Glutathione) Immunohistochemistry Oxidative Stress medicine.anatomical_structure Enzyme Reproductive Medicine Biochemistry chemistry embryonic structures Female Lipid Peroxidation Thioredoxin Oxidoreductases Oxidative stress Developmental Biology |
| Zdroj: | Placenta. 22(6) |
| ISSN: | 0143-4004 |
| Popis: | Recent studies have indicated that pre-eclampsia is closely associated with oxidative stress both in maternal circulation and in the placenta. Protein thiol/disulphide oxidoreductases, such as thioredoxin, glutaredoxin, and protein disulphide isomerase have recently been found to eliminate reactive oxygen species (ROS) and regenerate oxidatively damaged proteins. Protein thiol/disulphide oxidoreductases may also play a role in combating pre-eclampsia. In this study, we examined the accumulation of 4-hydroxy-2-nonenal (HNE)-modified proteins, which are markers of lipid peroxidation, in human placentae of normal and pre-eclamptic subjects. We also examined the protein levels of thioredoxin, glutaredoxin, and protein disulphide isomerase in placentae. Immunoblotting and immunohistochemistry showed that HNE-modified proteins accumulated to a greater extent in pre-eclamptic placentae than in normal placentae. In both normal and pre-eclamptic placentae, thioredoxin, glutaredoxin, and protein disulphide isomerase were detected in the trophoblasts of the floating villi. The levels of these proteins were increased approximately 2- to 3-fold in the pre-eclamptic placentae compared to the normal placentae. These results indicated that the pre-eclamptic placentae were exposed to oxidative stress and that the protein thiol/disulphide oxidoreductases were adaptively induced in pre-eclamptic placentae, suggesting possible roles for thioredoxin, glutaredoxin, and protein disulphide isomerase in protecting placental functions against oxidative stress caused by pre-eclampsia. |
| Databáze: | OpenAIRE |
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