Differences in oxidative stress dependence between gastric adenocarcinoma subtypes

Autor: Hiroshi Ohshima, Brigitte Bancel, Shinya Toyokuni, Brigitte Pignatelli, Jacques Estève, Jean-Christophe Souquet
Přispěvatelé: Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Adult
Male
Pathology
medicine.medical_specialty
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]
Nitric Oxide Synthase Type II
Adenocarcinoma
Biology
Nitric Oxide
medicine.disease_cause
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Stomach Neoplasms
medicine
Gastric mucosa
Carcinoma
Humans
Aged
Retrospective Studies
030304 developmental biology
Aged
80 and over
0303 health sciences
Carcinoma in situ
Nitrotyrosine
Gastroenterology
Deoxyguanine Nucleotides
DNA
Neoplasm
General Medicine
Middle Aged
medicine.disease
Immunohistochemistry
Neoplasm Proteins
3. Good health
Oxidative Stress
Gastric Cancer
medicine.anatomical_structure
chemistry
Gastric Mucosa
030220 oncology & carcinogenesis
Tyrosine
Female
Lymph
Precancerous Conditions
Biomarkers
Carcinoma in Situ
Oxidative stress
Zdroj: World Journal of Gastroenterology
World Journal of Gastroenterology, Baishideng Publishing Group Co. Limited, 2006, 12, pp.1005-1012
World Journal of Gastroenterology, 2006, 12, pp.1005-1012
ISSN: 1007-9327
Popis: AIM: To investigate the extent of oxidative stress in pre-neoplastic and neoplastic gastric mucosa in relation to their pathological criteria and histological subtypes. METHODS: A total of 104 gastric adenocarcinomas from 98 patients (88 infiltrative and 16 intraepithelial tumors) were assessed immunohistochemically for expression of iNOS and occurrence of nitrotyrosine (NTYR)-containing proteins and 8-hydroxy-2’-deoxyguanosine (8-OH-dG)-containing DNA, as markers of NO production and damages to protein and DNA. RESULTS: Tumor cells staining for iNOS, NTYR and 8-OH-dG were detected in 41%, 62% and 50% of infiltrative carcinoma, respectively. The three markers were shown for the first time in intraepithelial carcinoma. The expression of iNOS was significantly more frequent in tubular carcinoma (TC) compared to diffuse carcinoma (DC) (54% vs 18%; P = 0.008) or in polymorphous carcinoma (PolyC) (54% vs 21%; P = 0.04). NTYR staining was obviously more often found in TC than that in PolyC (72% vs 30%; P=0.03). There was a tendency towards a higher rate of iNOS staining when distant metastasis (pM) was present. In infiltrative TC, the presence of oxidative stress markers was not significantly correlated with histological grade, density of inflammation, the depth of infiltration (pT), lymph nodes dissemination (pN) and pathological stages (pTNM). CONCLUSION: The iNOS-oxidative pathway may play an important role in TC, but moderately in PolyC and DC. DNA oxidation and protein nitration occur in the three subtypes. Based on the significant differences of NTYR levels, TC and PolyC appear as two distinct subtypes.
Databáze: OpenAIRE
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