LncRNA DANCR attenuates brain microvascular endothelial cell damage induced by oxygen-glucose deprivation through regulating of miR-33a-5p/XBP1s
Autor: | Shuntong Kang, Qian Wu, Zhuohui Chen, Songfeng Zhao, Jingyan Sun, Bo Xiao, Mengqi Zhang, Xinhang Hu, Xinyi Lv, Mimi Tang, Tong Wu, Zhuolu Wang, Hui Ding |
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Rok vydání: | 2020 |
Předmět: |
X-Box Binding Protein 1
Untranslated region Aging XBP1 Angiogenesis miR-33a-5p Cell Movement microRNA ischemic stroke Humans Viability assay 3' Untranslated Regions Cell Proliferation brain microvascular endothelial cell X-box binding protein l splicing Chemistry Binding protein Brain Endothelial Cells Cell Biology Oxygen carbohydrates (lipids) Endothelial stem cell MicroRNAs Glucose Gene Expression Regulation nervous system Apoptosis Microvessels cardiovascular system Cancer research RNA Interference RNA Long Noncoding DANCR Research Paper |
Zdroj: | Aging (Albany NY) |
ISSN: | 1945-4589 |
Popis: | Brain microvascular endothelial cell (BMEC) survival and angiogenesis after ischemic stroke has great significance for improving the prognosis of stroke. Abnormal variants of lncRNAs are closely associated with stroke. In this study, we examined the effects and molecular mechanisms of differentiation antagonizing non-protein coding RNA (DANCR) on apoptosis, migration, and angiogenesis of oxygen-glucose deprivation (OGD)-treated BMECs. We found that DANCR expression significantly increased at 2, 4, 6, 8, and 10 h after OGD. DANCR overexpression promoted cell viability, migration, and angiogenesis in OGD-treated BMECs. Additionally, we found that X-box binding protein l splicing (XBP1s) expression was positively correlated with DANCR expression. DANCR overexpression promoted XBP1s expression in OGD-treated BMECs. Silenced XBP1s reversed the effect of DANCR in OGD-treated BMECs. Furthermore, we found that microRNA (miR)-33a-5p bound to DANCR and the 3'-UTR of XBP1. miR-33a-5p overexpression inhibited proliferation, migration, angiogenesis, and XBP1s expression in OGD-treated DANCR-overexpressing BMECs, reversing the protective effect of DANCR. Finally, we found that XBP1s expression promoted proliferation, migration, and angiogenesis, reversing the damaging effect of miR-33a-5p. In conclusion, DANCR enhanced survival and angiogenesis in OGD-treated BMECs through the miR-33a-5p/XBP1s axis. |
Databáze: | OpenAIRE |
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