Screening Gene Knockout Mice for Variation in Bone Mass: Analysis by μCT and Histomorphometry
| Autor: | Douglas J. Adams, Cheryl L. Ackert-Bicknell, Zhihua Wu, Seung-Hyun Hong, Caibin Zhang, David W. Rowe, Dong-Guk Shin, Gaven Garland, Dana. A. Godfrey, C. Renata Rydzik, John P. Sundberg, Li Chen |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Bone disease Databases Factual Genotype Information Management Endocrinology Diabetes and Metabolism Biology Severity of Illness Index Bone and Bones 03 medical and health sciences Distal femur Mice medicine Animals Femur Program Development Gene knockout Mice Knockout Sex Characteristics X-Ray Microtomography medicine.disease Spine Vertebra 030104 developmental biology medicine.anatomical_structure Phenotype Bone mass |
| Zdroj: | Current osteoporosis reports. 16(2) |
| ISSN: | 1544-2241 |
| Popis: | The international mouse phenotyping consortium (IMPC) is producing defined gene knockout mouse lines. Here, a phenotyping program is presented that is based on micro-computed tomography (μCT) assessment of distal femur and vertebra. Lines with significant variation undergo a computer-based bone histomorphometric analysis. Of the 220 lines examined to date, approximately 15% have a significant variation (high or low) by μCT, most of which are not identified by the IMPC screen. Significant dimorphism between the sexes and bone compartments adds to the complexity of the skeletal findings. The μCT information that is posted at www.bonebase.org can group KOMP lines with similar morphological features. The histological data is presented in a graphic form that associates the cellular features with a specific anatomic group. The web portal presents a bone-centric view appropriate for the skeletal biologist/clinician to organize and understand the large number of genes that can influence skeletal health. Cataloging the relative severity of each variant is the first step towards compiling the dataset necessary to appreciate the full polygenic basis of degenerative bone disease. |
| Databáze: | OpenAIRE |
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