Microsatellite markers for standardized genetic management of captive colonies of rhesus macaques (Macaca mulatta)
| Autor: | Andrea Von Dollen, Jennifer D. Kurushima, David Glenn Smith, Jeffrey Rogers, Betsy Ferguson, Ona Alminas, Philip C. Allen, Nicholas W. Lerche, Sreetharan Kanthaswamy |
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| Rok vydání: | 2006 |
| Předmět: |
Heterozygote
Population Breeding Biology Polymerase Chain Reaction Gene Frequency Genetic variation Multiplex polymerase chain reaction Animals Genetic variability Animal Husbandry education Ecology Evolution Behavior and Systematics DNA Primers Genetics Genetic diversity education.field_of_study Polymorphism Genetic Electrophoresis Capillary Genetic Variation Macaca mulatta Pedigree Specific Pathogen-Free Organisms Genetic marker Genetic structure Microsatellite Animal Science and Zoology Microsatellite Repeats |
| Zdroj: | American Journal of Primatology. 68:73-95 |
| ISSN: | 1098-2345 0275-2565 |
| Popis: | To preserve genetic variability and minimize genetic subdivision among captive Macaca mulatta at each of the U.S. National Institutes of Health (NIH)-sponsored regional research colonies, the genetic structure of each colony must be characterized. To compare population genetic and demographic parameters across colonies and generations, one standard panel of highly informative genetic markers is required. We assembled a core marker set of four multiplex polymerase chain reaction (PCR) panels comprising 15 autosomal short tandem repeat (STR) loci with high information content selected from existing panels of well-characterized markers that are currently used for parentage assessment and genetic management of rhesus macaques. We then assessed the effectiveness of these loci for providing high probabilities of individual identification and parentage resolution, and for estimating population genetic parameters that are useful for genetic management. |
| Databáze: | OpenAIRE |
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