The small nuclear ribonucleoprotein U1A interacts with NS5 from yellow fever virus
| Autor: | Paula Rahal, Carol S. Lutz, Danilo V. B. Duarte, Maurício Lacerda Nogueira, Sandro Roberto Valentini, João Alexandre Ribeiro Gonçalves Barbosa, Roberta Vieira de Morais Bronzoni, Maria C. F. S. Madrid, Cleslei Fernando Zanelli, Carolina Colombelli Pacca, Carolina Santacruz-Perez, Vanessa de Oliveira Arnoldi Pellegrini, Ana Carolina Viegas Carmo |
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| Rok vydání: | 2011 |
| Předmět: |
Polyadenylation
viruses Viral Nonstructural Proteins Polymerase Chain Reaction Ribonucleoprotein U1 Small Nuclear Conserved sequence Flaviviridae VP40 Two-Hybrid System Techniques Virology Chlorocebus aethiops Animals Humans Immunoprecipitation Protein Interaction Domains and Motifs Amino Acid Sequence Vero Cells Peptide sequence Conserved Sequence Ribonucleoprotein Binding Sites biology virus diseases General Medicine biology.organism_classification Flavivirus RNA Viral Yellow fever virus Small nuclear ribonucleoprotein HeLa Cells Protein Binding |
| Zdroj: | Archives of Virology. 156:931-938 |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | The flavivirus NS5 protein is one of the most important proteins of the replication complex, and cellular proteins can interact with it. This study shows for the first time that the yellow fever virus (YFV) NS5 protein is able to interact with U1A, a protein involved in splicing and polyadenylation. We confirmed this interaction by GST-pulldown assay and by co-immunoprecipitation in YFV-infected cells. A region between amino acids 368 and 448 was identified as the site of interaction of the NS5 protein with U1A. This region was conserved among some flaviviruses of medical importance. The implications of this interaction for flavivirus replication are discussed. |
| Databáze: | OpenAIRE |
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