Contrasting effects of Elg1-RFC and Ctf18-RFC inactivation in the absence of fully functional RFC in fission yeast
| Autor: | Iryna Charapitsa, Jiyoung Kim, Katie J. L. Mylonas, Stuart A. MacNeill, Fiona C. Gray, Kathryn Robertson |
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| Rok vydání: | 2005 |
| Předmět: |
DNA Replication
DNA Mutational Analysis Molecular Sequence Data Mutant Eukaryotic DNA replication medicine.disease_cause Article 03 medical and health sciences chemistry.chemical_compound Replication factor C Schizosaccharomyces Genetics medicine Amino Acid Sequence Replication Protein C Gene 030304 developmental biology 0303 health sciences Mutation biology 030302 biochemistry & molecular biology DNA replication Molecular biology Protein Structure Tertiary Proliferating cell nuclear antigen DNA-Binding Proteins Protein Subunits chemistry biology.protein Genes Lethal Schizosaccharomyces pombe Proteins Carrier Proteins Gene Deletion DNA Transcription Factors |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gki728 |
| Popis: | Proliferating cell nuclear antigen loading onto DNA by replication factor C (RFC) is a key step in eukaryotic DNA replication and repair processes. In this study, the C-terminal domain (CTD) of the large subunit of fission yeast RFC is shown to be essential for its function in vivo. Cells carrying a temperature-sensitive mutation in the CTD, rfc1-44, arrest with incompletely replicated chromosomes, are sensitive to DNA damaging agents, are synthetically lethal with other DNA replication mutants, and can be suppressed by mutations in rfc5. To assess the contribution of the RFC-like complexes Elg-1-RFC and Ctf18-RFC to the viability of rfc1-44, genes encoding the large subunits of these complexes have been deleted and over-expressed. Inactivation of Ctf18-RFC by the deletion of ctf18 + , dcc1 + or ctf8 + is lethal in an rfc1-44 background showing that full Ctf18-RFC function is required in the absence of fully functional RFC. In contrast, rfc1-44 elg1Δ cells are viable and overproduction of Elg1 in rfc1-44 is lethal, suggesting that Elg1-RFC plays a negative role when RFC function is inhibited. Consistent with this, the deletion of elg1 + is shown to restore viability to rfc1-44 ctf18Δ cells. |
| Databáze: | OpenAIRE |
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