Sperm-oocyte signaling: the role of IZUMO1R and CD9 in PTK2B activation and actin remodeling at the sperm binding site†
| Autor: | Xiaoman Hong, Huizhen Wang, William H. Kinsey |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male endocrine system Mice Transgenic Receptors Cell Surface Biology Tetraspanin 29 03 medical and health sciences Mice medicine Animals Protein kinase A Actin reproductive and urinary physiology Sperm-Ovum Interactions PTK2B 030102 biochemistry & molecular biology urogenital system Actin remodeling Cell Biology General Medicine Oocyte Sperm Spermatozoa Cell biology 030104 developmental biology medicine.anatomical_structure Focal Adhesion Kinase 2 Reproductive Medicine embryonic structures Oocytes Gamete Phosphorylation Signal Transduction Research Article |
| Zdroj: | Biol Reprod |
| ISSN: | 1529-7268 |
| Popis: | Sperm–oocyte binding initiates an outside-in signaling event in the mouse oocyte that triggers recruitment and activation of the cytosolic protein kinase PTK2B in the cortex underlying the bound sperm. While not involved in gamete fusion, PTK2B activity promotes actin remodeling events important during sperm incorporation. However, the mechanism by which sperm–oocyte binding activates PTK2B is unknown, and the present study examined the possibility that sperm interaction with specific oocyte surface proteins plays an important role in PTK2B activation. Imaging studies revealed that as IZUMO1R and CD9 became concentrated at the sperm binding site, activated (phosphorylated) PTK2B accumulated in the cortex underlying the sperm head and in microvilli partially encircling the sperm head. In order to determine whether IZUMO1R and/or CD9 played a significant role in PTK2B recruitment and activation at the sperm binding site, the ability of oocytes null for Izumo1r or Cd9, to initiate an increase in PTK2B content and activation was tested. The results revealed that IZUMO1R played a minor role in PTK2B activation and had no effect on actin remodeling; however, CD9 played a very significant role in PTK2B activation and subsequent actin remodeling at the sperm binding site. These findings suggest the possibility that interaction of sperm surface proteins with CD9 or CD9-associated oocyte proteins triggers PTK2B activation at the sperm binding site.Summary sentenceSperm binding triggers PTK2B activation in the oocyte through a CD9-dependent mechanism. |
| Databáze: | OpenAIRE |
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