Production of lipid mediators in mastitic milk of cow

Autor:	Shota Fujii, Tatsuro Nakamura, Tomotsugu Takahashi, Koji Kobayashi, Akane Hayashi, Maiko Endo, Takahisa Murata, Miki Sakatani
Rok vydání:	2019
Předmět:	Docosahexaenoic Acids Lipoxygenase Leukotriene B4 Dinoprostone 03 medical and health sciences chemistry.chemical_compound medicine Animals Food science Mastitis Bovine 030304 developmental biology chemistry.chemical_classification 0303 health sciences Leukotriene Arachidonic Acid biology Prostaglandin D2 0402 animal and dairy science food and beverages 04 agricultural and veterinary sciences General Medicine Lipid signaling medicine.disease 040201 dairy & animal science Eicosapentaenoic acid Mastitis Milk Eicosapentaenoic Acid chemistry Prostaglandin-Endoperoxide Synthases Docosahexaenoic acid Fatty Acids Unsaturated biology.protein Cattle Female lipids (amino acids peptides and proteins) Arachidonic acid General Agricultural and Biological Sciences Biomarkers Polyunsaturated fatty acid
Zdroj:	SC10202006170003 NARO成果DBa
ISSN:	1740-0929 1344-3941
DOI:	10.1111/asj.13222
Popis:	Bovine mastitis is one of the most prevalent and costly diseases in the dairy industry. Lipid mediators are signaling molecules which coordinately and intricately modulate inflammation. They are produced from polyunsaturated fatty acids (PUFAs) in the cellular membrane via several enzymes including cyclooxygenase (COX) and lipoxygenase (LOX). In the present study, we performed comprehensive analysis of lipid production in milk obtained from clinical or subclinical mastitic cows using liquid chromatography/mass spectrometry. We detected 26, 24, and 40 kinds of lipid constantly in healthy, subclinical, and clinical mastitic milk, respectively. In clinical mastitic milk, the amount of a major n-6 PUFA, arachidonic acid (AA), tended to increase, whereas amounts of major n-3 PUFAs, eicosapentaenoic acid and docosahexaenoic acid, tended to decrease. The amounts of several AA-derived lipids including COX-catalyzed prostaglandin (PG) D2 and PGE2 , and LOX-catalyzed leukotriene (LT) B4 were increased in clinical mastitic milk. Although subclinical mastitic milk represented similar trend of lipid production to healthy milk, amounts of several lipids such as LTD4 , 14,15-dihydroxyeicosatrienoic acid, and 14-epoxyeicosatrienoic acid changed. These findings would be helpful for better understanding of mastitis pathology and give us some insights to develop a new diagnostic and therapeutic strategy.
Databáze:	OpenAIRE
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