In vivo germination of Bacillus anthracis spores during murine cutaneous infection
| Autor: | Marie Moya-Nilges, Jean-Philippe Corre, Michèle Mock, Jean-Claude Sirard, Agnès Fouet, Ian J. Glomski, Pierre L. Goossens, Alejandro Piris-Gimenez, Grégory Jouvion |
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| Přispěvatelé: | Toxines et Pathogénie Bactérienne, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Histopathologie humaine et Modèles animaux, Institut Pasteur [Paris], Institut Cochin (IC UM3 (UMR 8104 / U1016)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Microbiology, Immunology and Cancer Biology, University of Virginia [Charlottesville], Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Centre d’Infection et d’Immunité de Lille (CIIL) - U1019 - UMR 8204 ( CIIL ), Réseau International des Instituts Pasteur ( RIIP ) -Réseau International des Instituts Pasteur ( RIIP ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -IFR142-Université de Lille-Centre National de la Recherche Scientifique ( CNRS ), Sirard, Jean-Claude, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), University of Virginia |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
MESH: Skin Diseases
Bacterial MESH: Spleen Colony Count Microbial Bacterial growth Mice MESH: Spores Bacterial MESH : Skin Diseases Bacterial Spore germination Immunology and Allergy [ SDV.IMM ] Life Sciences [q-bio]/Immunology MESH : Female MESH: Animals MESH : Host-Pathogen Interactions MESH : Lymphoid Tissue Spores Bacterial 0303 health sciences biology MESH : Spores Bacterial MESH : Bacillus anthracis MESH : Anthrax Bacillus anthracis MESH: Bacillus anthracis Infectious Diseases medicine.anatomical_structure Liver Germination Host-Pathogen Interactions [SDV.IMM]Life Sciences [q-bio]/Immunology Female MESH : Guinea Pigs MESH : Spleen [SDV.IMM] Life Sciences [q-bio]/Immunology Lymphoid Tissue MESH : Colony Count Microbial Guinea Pigs Spleen Microbiology MESH: Guinea Pigs Anthrax 03 medical and health sciences In vivo MESH : Mice medicine MESH: Anthrax Animals MESH: Mice MESH: Colony Count Microbial 030304 developmental biology 030306 microbiology Inoculation fungi MESH: Host-Pathogen Interactions MESH : Liver Skin Diseases Bacterial biology.organism_classification MESH : Disease Models Animal Spore Disease Models Animal MESH: Lymphoid Tissue MESH : Animals MESH: Disease Models Animal MESH: Female MESH: Liver |
| Zdroj: | Journal of Infectious Diseases Journal of Infectious Diseases, Oxford University Press (OUP), 2013, 207 (3), pp.450-7. ⟨10.1093/infdis/jis686⟩ Journal of Infectious Diseases, Oxford University Press (OUP), 2013, 207 (3), pp.450-7. 〈10.1093/infdis/jis686〉 Journal of Infectious Diseases, 2013, 207 (3), pp.450-7. ⟨10.1093/infdis/jis686⟩ |
| ISSN: | 0022-1899 1537-6613 |
| DOI: | 10.1093/infdis/jis686⟩ |
| Popis: | International audience; BACKGROUND: Germination is a key step for successful Bacillus anthracis colonization and systemic dissemination. Few data are available on spore germination in vivo, and the necessity of spore and host cell interactions to initiate germination is unclear. METHODS: To investigate the early interactions between B. anthracis spores and cutaneous tissue, spores were inoculated in an intraperitoneal cell-free device in guinea pigs or into the pinna of mice. Germination and bacterial growth were analyzed through colony-forming unit enumeration and electron microscopy. RESULTS: In the guinea pig model, germination occurred in vivo in the absence of cell contact. Similarly, in the mouse ear, germination started within 15 minutes after inoculation, and germinating spores were found in the absence of surrounding cells. Germination was not observed in macrophage-rich draining lymph nodes, liver, and spleen. Edema and lethal toxin production were not required for germination, as a toxin-deficient strain was as effective as a Sterne-like strain. B. anthracis growth was locally controlled for 6 hours. CONCLUSIONS: Spore germination involving no cell interactions can occur in vivo, suggesting that diffusible germinants or other signals appear sufficient. Different host tissues display drastic differences in germination-triggering capacity. Initial control of bacterial growth suggests a therapeutic means to exploit host innate defenses to hinder B. anthracis colonization. |
| Databáze: | OpenAIRE |
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