Effects of calorie restriction on thymocyte growth, death and maturation
| Autor: | David B. Klug, Ellen R. Richie, Heather L. Poetschke, Stephen D. Hursting, Thomas T.Y. Wang, Susan N. Perkins |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male Cancer Research medicine.medical_specialty Programmed cell death Lymphoma Ratón T-Lymphocytes Calorie restriction Population Spleen Thymus Gland Biology CD8-Positive T-Lymphocytes Mice Antigens CD T-Lymphocyte Subsets Internal medicine medicine Animals education education.field_of_study Cell Death Cell growth Body Weight Cell Differentiation General Medicine Thymus Neoplasms Flow Cytometry Mice Inbred C57BL Thymocyte Endocrinology medicine.anatomical_structure Energy Intake CD8 |
| Zdroj: | Carcinogenesis. 21(11) |
| ISSN: | 0143-3334 |
| Popis: | We previously reported that calorie restriction (CR) significantly delays the spontaneous development of thymic lymphomas and other neoplasms in p53-deficient mice and their wild-type littermates. The purpose of the present study was to further characterize the anti-lymphoma effects of CR by assessing thymocyte growth, death and maturation in response to acute (6 day) and chronic (28 day) CR regimens. Male C57BL/6J mice fed a CR diet (restricted to 60% of control ad libitum intake) for 6 days displayed a severe reduction in thymic size and cellularity, as well as a decrease in splenic size and cellularity; these declines were sustained through 28 days of CR. Mice maintained on a CR diet for 28 days also displayed a significant depletion in the cell numbers of all four major thymocyte subsets defined by CD4 and CD8 expression. Analysis within the immature CD4(-)8(-) thymocyte subset further revealed an alteration in normal CD44 and CD25 subset distribution. In particular, CR for 28 days resulted in a significant decrease in the percentage of the proliferative CD44(-)25(-) subset. In addition, a significant increase in the percentage of the early, pro-T cell CD44(+)25(-) population was detected, indicative of a CR-induced delay in thymocyte maturation. Taken together, these findings suggest that CR suppresses (through several putative mechanisms) lymphomagenesis by reducing the pool of immature thymocytes that constitute the lymphoma-susceptible subpopulation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|