Therapeutic effects of triptolide via the inhibition of IL-1β expression in a mouse model of ulcerative colitis
| Autor: | Bin Zhang, Guoxiong Zhou, Lishuai Qu, Chen Gong, Hai-Qin Chen, Xiao-Ling Ding, Wei Cao, Hai-Feng Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research medicine.medical_specialty Pathology interleukin-1β medicine.medical_treatment 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immunology and Microbiology (miscellaneous) Western blot Internal medicine medicine Saline Dexamethasone ulcerative colitis medicine.diagnostic_test Oncogene treatment business.industry Therapeutic effect General Medicine Articles Triptolide medicine.disease Ulcerative colitis 030104 developmental biology Endocrinology chemistry Apoptosis 030220 oncology & carcinogenesis triptolide business medicine.drug |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| Popis: | The present study aimed to investigate the effect of triptolide (TL) on ulcerative colitis (UC) and explore the potential association between the therapeutic effects of TL and IL-1β expression using a 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS)-induced mouse model to simulate human UC. A total of 70 BALB/c female mice were randomly allocated into seven equal groups: Group A, blank control; group B, normal saline injection; group C, propylene glycol injection; group D (TL1), 0.2 mg/kg TL; group E (TL2), 0.4 mg/kg TL; group F (TL3), 0.6 mg/kg TL; and group G, dexamethasone injection. Mice activity, diet and stool characteristics were recorded daily. Mice were sacrificed by cervical dislocation on day 8, and disease activity indices, colon tissue histological scores and colonic histopathological scores were subsequently calculated. Serum levels of IL-1β were evaluated by enzyme-linked immunosorbent assay, and IL-1β expression levels were examined by reverse transcription-quantitative polymerase chain reaction with colonic mucosa specimen at the gene level and western blot analysis at the protein level. The IL-1β mRNA and protein expression levels were significantly elevated in the normal saline injection and propylene glycol injection groups compared with the blank control group and (P |
| Databáze: | OpenAIRE |
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