How low can you go? Pushing the limits of low-input ChIP-seq
| Autor: | Gregor D. Gilfillan, John Arne Dahl |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Chromatin Immunoprecipitation Low input Microfluidics High-Throughput Nucleotide Sequencing General Medicine Computational biology Biology Chip Biochemistry 03 medical and health sciences 030104 developmental biology Genetics Animals Humans Molecular Biology Chromatin Immunoprecipitation Sequencing Gene Library |
| Zdroj: | Briefings in functional genomics. 17(2) |
| ISSN: | 2041-2657 |
| Popis: | In the past decade, chromatin immunoprecipitation sequencing (ChIP-seq) has emerged as the dominant technique for those wishing to perform genome-wide protein:DNA profiling. Owing to the tissue- and cell-type-specific nature of epigenetic marks, the field has been driven towards obtaining data from ever-lower cell numbers. In this review, we focus on the methodological developments that have lowered input requirements and the biological findings they have enabled, as we strive towards the ultimate goal of robust single-cell ChIP-seq. |
| Databáze: | OpenAIRE |
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