Mechanism of citrinin-induced dysfunction of mitochondria. II. Effect on respiration, enzyme activities, and membrane potential of liver mitochondria
| Autor: | Ma. Lúcia W. Klüppel, Generoso M. Chagas, Annibal P. Campello, Ma. Bengna M. Oliveira |
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| Rok vydání: | 1992 |
| Předmět: |
animal structures
Clinical Biochemistry Respiratory chain Mitochondria Liver Biology Mitochondrion Biochemistry Membrane Potentials chemistry.chemical_compound Oxygen Consumption medicine Animals Membrane potential chemistry.chemical_classification Depolarization Cell Biology General Medicine Citrinin Rats Enzyme Activation Enzyme Mechanism of action chemistry Phosphorylation medicine.symptom Protons Energy Metabolism |
| Zdroj: | Cell biochemistry and function. 10(3) |
| ISSN: | 0263-6484 |
| Popis: | The mycotoxin citrinin, depressed the phosphorylation efficiency of liver mitochondria as deduced from a decrease of respiratory coefficient and of the ADP/O ratio. Citrinin (1.0 mM) inhibited some enzymes linked to the respiratory chain, namely NADH oxidase and NADH cytochrome c reductase involved with complex I. The activities of enzymes related with other enzymatic complexes of the respiratory chain were either unaffected or enhanced. ATPase activity was inhibited by the mycotoxin. Malate, glutamate, and 2-oxoglutarate dehydrogenases were also inhibited. The transmembrane potential (delta psi), developed by energized mitochondria and depolarization on the addition of ADP, was decreased. The results suggest that citrinin promotes a partial dissipation of the transmembrane potential, different from that resulting from a classical uncoupler such as 2,4-dinitrophenol. |
| Databáze: | OpenAIRE |
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