Increased free radical production in hypertension due to increased expression of the NADPH oxidase subunit p22phox in lymphoblast cell lines
Autor: | Paulene A. Quinn, Leong L. Ng, Lee, Sonja C. Jennings, Wong Rk, Pettit Ai |
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Rok vydání: | 2002 |
Předmět: |
Male
medicine.medical_specialty Free Radicals Physiology Cell Line Luminol chemistry.chemical_compound Western blot Internal medicine Internal Medicine medicine Humans Lymphocytes chemistry.chemical_classification Reactive oxygen species Oxidase test Arachidonic Acid NADPH oxidase medicine.diagnostic_test biology business.industry Lymphoblast NADPH Dehydrogenase Membrane Transport Proteins NADPH Oxidases Middle Aged Phosphoproteins Endocrinology Enzyme chemistry Hypertension Luminescent Measurements biology.protein Tetradecanoylphorbol Acetate Female Arachidonic acid Cardiology and Cardiovascular Medicine business |
Zdroj: | Journal of Hypertension. 20:677-683 |
ISSN: | 0263-6352 |
DOI: | 10.1097/00004872-200204000-00025 |
Popis: | OBJECTIVES To confirm increased production of reactive oxygen species (ROS) in hypertension, to demonstrate the source of ROS and to analyse NADPH oxidase subcomponent expression in hypertension. DESIGN A lymphoblast model was used, as this has previously been used in the study of hypertension and of NADPH oxidase. Chemiluminescence (CL) was chosen to assay ROS production, as it is simple and sensitive. METHODS Lymphocytes from 12 hypertensive patients (HT), and 12 age- and sex-matched normotensive (NT) subjects, were immortalized. Luminol, isoluminol and Cypridina luciferin analogue (CLA) CL were used to assay ROS production. NADPH oxidase subunits were measured by Western blot analysis. RESULTS Stimulation with 50 micromol/l arachidonic acid (AA) resulted in increased ROS production in HT cell lines with luminol, CLA and isoluminol CL. Stimulation with 500 nmol/l 12-O-tetradecanoylphorbol-13-acetate (TPA) produced a detectable increase in HT ROS production with luminol and with CLA, whereas there was no significant difference with isoluminol. The ROS production was abolished by diphenyleneiodonium chloride (DPI) but not by rotenone, indicating that a non-mitochondrial flavoprotein such as NADPH oxidase is the source of ROS. Analysis of NADPH oxidase subcomponents revealed an increase in p22(phox) in HT subjects. CONCLUSIONS We have shown there is increased ROS production in lymphoblasts derived from hypertensive subjects, probably originating from NADPH oxidase. As the ROS production persists in transformed cells, this suggests a genetic predisposition to increased ROS production. Increased expression of p22(phox) in HT lymphoblasts may account for some of the increased ROS. |
Databáze: | OpenAIRE |
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