Embryonic stem cell-related miRNAs are involved in differentiation of pluripotent cells originating from the germ line
| Autor: | Marieke Wolf, Athanasios Zovoilis, Angeliki Pantazi, Anna Holubowska, Colin L. Stewart, Ulrich Zechner, Gabriela Salinas Riester, Lukasz Smorag, Lennart Opitz, Wolfgang Engel |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Pluripotent Stem Cells Embryology Embryonic Germ Cells Adult Germline Stem Cells Cellular differentiation Biology 03 medical and health sciences Mice 0302 clinical medicine Genetics medicine Animals Cell Lineage Computer Simulation Induced pluripotent stem cell Molecular Biology Embryonic Stem Cells 030304 developmental biology 0303 health sciences Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Multipotent Stem Cells Obstetrics and Gynecology Cell Differentiation Cell Biology Embryonic stem cell Cell biology MicroRNAs medicine.anatomical_structure Germ Cells Reproductive Medicine Germ line development Stem cell 030217 neurology & neurosurgery Germ cell Biomarkers Developmental Biology |
| Zdroj: | Molecular human reproduction. 16(11) |
| ISSN: | 1460-2407 |
| Popis: | Cells originating from the germ cell lineage retain the remarkable property under special culture conditions to give rise to cells with embryonic stem cell (ESC) properties, such as the multipotent adult germline stem cells (maGSCs) derived from adult mouse testis. To get an insight into the mechanisms that control pluripotency and differentiation in these cells, we studied how differences observed during in vitro differentiation between ESCs and maGSCs are associated with differences at the level of microRNAs (miRNAs). In this work, we provide for a first time a connection between germ cell origin of maGSCs and their specific miRNA expression profile. We found that maGSCs express higher levels of germ cell markers characteristic for primordial germ cells (PGCs) and spermatogonia compared with ESCs. Retained expression of miR-290 cluster has been previously reported in maGSCs during differentiation and it was associated with higher Oct-4 levels. Here, we show that this property is also shared by another pluripotent cell line originating from the germ line, the embryonic germ cells. In addition, we provide proof that the specific miRNA expression profile of maGSCs has an impact on their differentiation potential. Low levels of miR-302 in maGSCs during the first 10 days of leukaemia inhibitory factor deprivation are shown to be necessary for the maintenance of high levels of early germ cell markers. |
| Databáze: | OpenAIRE |
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