Polyclonal antisera production by immunization with mixed cell antigens of different rhizobial species
| Autor: | Y. D. Gaur, N. Boonkerd, Padma Somasegaran, Heinz J. Hoben |
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| Rok vydání: | 1994 |
| Předmět: |
Antiserum
biology Physiology General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Applied Microbiology and Biotechnology Microbiology Agglutination (biology) Antigen Immunization Polyclonal antibodies biology.protein bacteria Rhizobium Antibody Biotechnology Bradyrhizobium japonicum |
| Zdroj: | World journal of microbiologybiotechnology. 10(5) |
| ISSN: | 0959-3993 |
| Popis: | Somatic antigens of Bradyrhizobium japonicum, Rhizobium sp. (Cicer arietinum) and Rhizobium sp. (Leucaena leucocephala) were prepared as standard, single-species type from cultured cells. Equal numbers of the cells of these rhizobia were then combined to obtain a mixed-rhizobial-species antigen preparation. Rabbits were immunized either with the standard, single-species type or with the mixed-rhizobial-species antigen preparations. The antisera developed from the mixed antigen immunization contained antibodies for all three rhizobial species, detectable at agglutination titres of over 800. The mixed-rhizobial-species antisera were made species specific by cross-absorption. The cross-absorbed and the mixed-rhizobial-species antisera were generally similar in quality for strain identification by agglutination, fluorescent antibodies, immunoblot and ELISA. A 66% reduction in cost was estimated for the production of antisera by immunization with mixed-rhizobial-species antigen. |
| Databáze: | OpenAIRE |
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