Tear fluid analysis in patients with primary Sjögren's syndrome using lectin probes. A comparative study of patients with primary Sjögren's syndrome, patients with other immune inflammatory connective tissue diseases and controls
| Autor: | Kirsten B. Bjerrum |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Blotting Western Connective tissue Immune system Lectins Medicine Humans Eye Proteins Polyacrylamide gel electrophoresis Aged Glycoproteins chemistry.chemical_classification Aged 80 and over biology business.industry Lectin Middle Aged Staining Blot Molecular Weight Ophthalmology medicine.anatomical_structure Sjogren's Syndrome chemistry Tears Immunology biology.protein Electrophoresis Polyacrylamide Gel Female business Glycoprotein |
| Zdroj: | Acta ophthalmologica Scandinavica. 77(1) |
| ISSN: | 1395-3907 |
| Popis: | Purpose To study and compare the glycoprotein composition of tear fluid from patients with primary Sjogren's syndrome, patients with other connective tissue diseases and control individuals. Methods SDS polyacrylamide gel electrophoresis followed by Coomassie staining or Western blotting and overlaying with ten different lectins. Results The frequency of many glycoprotein bands discovered was significantly decreased in primary Sjogren's syndrome compared with controls and patients with other connective tissue diseases. The reduction was most pronounced in the 14-68 kDa molecular weight range. Conclusions A decrease in the production rate and/or a change in the glycosylation pattern of the tear glycoproteins was found in patients with primary Sjogren's syndrome. The changes discovered, however, were not specific enough to be useful as a diagnostic test. |
| Databáze: | OpenAIRE |
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