Lycium barbarum polysaccharides inhibit ischemia/reperfusion-induced myocardial injury via the Nrf2 antioxidant pathway
| Autor: | Hao Yu Gong, Yi Lu, Lei Lei He, Gong Xiao Zhao, Zheng Wang, Jin Jun Liu, Chun Fang Li, Abdoulaye Issotina Zibrila, Bai Chun Niu, Lynn Soong, Jing Ning Xu |
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| Rok vydání: | 2021 |
| Předmět: |
Health
Toxicology and Mutagenesis 010501 environmental sciences Pharmacology Toxicology medicine.disease_cause 01 natural sciences Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine RA1190-1270 Lactate dehydrogenase medicine 0105 earth and related environmental sciences chemistry.chemical_classification Reactive oxygen species biology Glutathione peroxidase Regular Article Malondialdehyde Heme oxygenase Ischemia reperfusion Myocardial infarction Lycium barbarum polysaccharides chemistry Oxidative stress Toxicology. Poisons biology.protein Creatine kinase 030217 neurology & neurosurgery |
| Zdroj: | Toxicology Reports, Vol 8, Iss, Pp 657-667 (2021) Toxicology Reports |
| ISSN: | 2214-7500 |
| Popis: | Graphical abstract LBP attenuated myocardial ischemia/reperfusion injury by restoring redox status, ultimately improving cardiac function. CAT: catalase; GPX: glutathione peroxidase; HO1: heme oxygenase 1; IL-6: interleukin-6; I/R: ischemia/reperfusion; LBP: Lycium barbarum polysaccharides; Nrf2: nuclear factor erythroid 2-related factor 2; NQO1: NADPH dehydrogenase quinone 1; ROS: reactive oxygen species; SOD: superoxide dismutase; TNF-α: tumor necrosis factor α. Highlights • LBP attenuated myocardial oxidative stress and apoptosis induced by I/R. • LBP improved cardiac function by reducing oxidative stress and apoptosis. • LBP ameliorated myocardial I/R injury via activation the Nrf2 signal pathway. Oxidative stress is considered to be one of main pathophysiological mechanisms in myocardial ischemia/reperfusion (I/R) injury. Lycium barbarum polysaccharides (LBP), the main ingredient of Lycium barbarum, have potential antioxidant activity. We aimed to investigate the effects of LBP on myocardial I/R injury and explore the underlying mechanisms. Myocardial I/R group was treated with or without LBP to evaluate oxidative stress markers and the role of Nrf2 signal pathway. Our results showed that I/R increased infarct size and the activities of creatine kinase (CK) and lactate dehydrogenase (LDH) when compared with control group. Meanwhile, the levels of reactive oxygen species (ROS), malondialdehyde (MDA), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were enhanced and the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) were decreased. These changes were associated with a significant increase in myocardial apoptosis, ultimately leading to cardiac dysfunction. LBP reduced infarct size (38.4 ± 2 % versus 19.4 ± 1.8 %, p < 0.05), CK and LDH activities and myocardial apoptotic index. Meanwhile, LBP suppressed the production of ROS and restored redox status. Additionally, LBP increased protein level of nuclear Nrf2 in vivo (2.1 ± 0.3 versus 3.8 ± 0.4, p < 0.05) and in vitro (1.9 ± 0.2 versus 3.8 ± 0.1, p < 0.05) and subsequently upregulated heme oxygenase 1 and NADPH dehydrogenase quinone 1 compared to I/R group. Interestingly, Nrf2 siRNA abolished the protective effects of LBP. LBP suppressed oxidative stress damage and attenuated cardiac dysfunction induced by I/R via activation of the Nrf2 antioxidant signal pathway. |
| Databáze: | OpenAIRE |
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