Clinical-like cryotherapy improves footprint patterns and reduces synovial inflammation in a rat model of post-traumatic knee osteoarthritis
| Autor: | Germanna Medeiros Barbosa, Fernando Q. Cunha, Tania F. Salvini, Jonathan Emanuel Cunha, Fernando Silva Ramalho, Paula Aiello Tomé de Souza Castro, Francisco Fábio Bezerra de Oliveira, Lizandra Botaro Martinho, Thiago M. Cunha |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Anterior cruciate ligament medicine.medical_treatment lcsh:Medicine Cryotherapy Cartilage metabolism Osteoarthritis Placebo Gastroenterology Article 03 medical and health sciences 0302 clinical medicine Cell Movement Fibrosis Internal medicine Synovial Fluid Leukocytes medicine Animals Synovial fluid Rats Wistar lcsh:Science Gait Inflammation 030203 arthritis & rheumatology Multidisciplinary business.industry Interleukins lcsh:R Synovial Membrane Osteoarthritis Knee JOELHO medicine.disease Hindlimb Rats Disease Models Animal Cartilage medicine.anatomical_structure Wounds and Injuries lcsh:Q Synovial membrane Skin Temperature business 030217 neurology & neurosurgery |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019) |
| Popis: | Cryotherapy is a non-pharmacological treatment commonly used to control inflammation and improve function after acute traumas. However, there are no definitive findings about its effects on chronic joint diseases such as knee osteoarthritis (KOA). The aim of this study was to investigate the effects of clinical-like cryotherapy on functional impairment and synovial inflammation in a rat model of KOA generated by anterior cruciate ligament transection (ACLT). Thirty-two male Wistar rats were randomly divided into four groups (n = 8/group): Control, KOA, KOA + Cryotherapy and KOA + Placebo. The last two groups were submitted to the relevant interventions twice a day for five days (61 to 65), with each session lasting 20 min. Gait test, skin temperature, thermal response threshold and joint swelling were assessed in all groups before ACLT surgery, and pre (60th day) and post (66th day) intervention protocols. On day 66, the animals were euthanized and exsanguinated to remove the synovial membrane for histopathological examination and synovial fluid to determine the leukocyte count and cytokine concentration. After the intervention period (66th day), footprint area only increased in the KOA + Cryotherapy group (P = 0.004; 14%) when compared to KOA and KOA + Placebo, but did not differ from controls. Cryotherapy lowered the synovial fluid leukocyte count (P P |
| Databáze: | OpenAIRE |
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