Comparative proteomic analysis of rat juvenile and adult dura
| Autor: | Zhe Peng, Tao Song, Lai Gui, Bao-Sheng Chen, Nan Bi |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Electrophoresis
Proteomics Aging Two-dimensional gel electrophoresis Regeneration (biology) Dura mater Blotting Western Proteins Vimentin General Medicine Anatomy Biology Tropomyosin Mass Spectrometry Rats Rats Sprague-Dawley Andrology Blot medicine.anatomical_structure Proteome medicine biology.protein Animals Juvenile Dura Mater |
| Zdroj: | Chinese Medical Journal. 121:1815-1820 |
| ISSN: | 0366-6999 |
| DOI: | 10.1097/00029330-200809020-00012 |
| Popis: | BACKGROUND It has been widely observed that infants and young children can reossify large calvarial defects when they are younger than 2 years of age; afterwards, they lose this regenerative potential. Previous studies have implicated that the dura mater serves as a key regulator of calvarial regeneration. However, the molecular mechanism of calvarial reossification remains elusive. METHODS In order to identify the proteins that may participate in this process, we performed a proteome-wide comparison of the protein expression levels of immature and mature dura using 2D electrophoresis and MALDI-TOF mass spectrometry. The Western blotting was used to verify the results of the 2D electrophoresis/MALDI-TOF mass spectrometry. RESULTS Eleven proteins were found to express with significant differences in the immature and the mature dura. Among them, the emergence of vimentin, tropomyosin, beta-actin and gamma-actin were further confirmed by the Western blotting analysis. CONCLUSION The proteins and proteomic profiles provide a better understanding of the molecular mechanism of calvarial regeneration. |
| Databáze: | OpenAIRE |
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