Reversing charges or how to improve Wharton's jelly mesenchymal stem cells culture on polyelectrolyte multilayer films
| Autor: | Jacqueline Beroud, Monique Gentils, Emilie Velot, P Labrude, Patrick Menu, Halima Kerdjoudj, Hassan Rammal |
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| Přispěvatelé: | Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL), Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Polymers
Cell Culture Techniques Antigens CD34 Biocompatible Materials MESH: Flow Cytometry 02 engineering and technology Expanded polytetrafluoroethylene chemistry.chemical_compound Coated Materials Biocompatible MESH: Coated Materials Biocompatible MESH: Biocompatible Materials Wharton's jelly Electrochemistry Polyamines 5'-Nucleotidase MESH: Antigens CD MESH: Receptors Cell Surface 0303 health sciences Endoglin MESH: Polystyrenes General Medicine Adhesion Flow Cytometry 021001 nanoscience & nanotechnology Polyelectrolytes Polyelectrolyte MESH: Glass MESH: Polymers Phenotype 0210 nano-technology Surface Properties Biomedical Engineering Receptors Cell Surface MESH: Antigens CD45 GPI-Linked Proteins MESH: Phenotype Allylamine MESH: Cell Adhesion Biomaterials 03 medical and health sciences Antigens CD Cations MESH: Cell Proliferation Cell Adhesion Humans MESH: Cell Shape [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] MESH: Cations Cell Shape MESH: Polyamines Cell Proliferation 030304 developmental biology MESH: Surface Properties MESH: Cell Culture Techniques MESH: Electrochemistry MESH: Humans Mesenchymal stem cell Mesenchymal Stem Cells [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology MESH: Antigens CD34 chemistry Biophysics MESH: Mesenchymal Stromal Cells Leukocyte Common Antigens Polystyrenes Thy-1 Antigens MESH: Antigens Thy-1 Glass MESH: GPI-Linked Proteins MESH: 5'-Nucleotidase MESH: Endoglin [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology Biomedical engineering |
| Zdroj: | Bio-Medical Materials and Engineering Bio-Medical Materials and Engineering, IOS Press, 2013, 23 (4), pp.299-309. ⟨10.3233/BME-130754⟩ |
| ISSN: | 0959-2989 1878-3619 |
| DOI: | 10.3233/BME-130754⟩ |
| Popis: | Polyelectrolyte multilayer (PEMs) films made of poly(allylamine hydrochloride) (PAH) as polycation and poly(styrene sulfonate) (PSS) as polyanion, with a PAH ending layer, can be used as a coating in order to improve the anti-thrombogenicity and patency of vascular grafts in vascular engineering field. They induce strong adhesion of mature endothelial cells on glass, expanded polytetrafluoroethylene and cryopreserved arteries. Despite their outstanding effect on mature and progenitor endothelial cells, PEMs ending with PAH showed a poor outcome on Wharton's jelly mesenchymal stem cells (WJ-MSCs) culture.The aim of this work was to examine the influence of the ending charge of PEMs on WJ-MSCs behavior.WJ-MSCs amplified until the 3rd passage were seeded and cultured on (PAH-PSS)3-PAH and on (PAH-PSS)4 coated glass for 10 days. Stem cell phenotype was checked by flow cytometry and cell morphology was followed by bright field microscopy.Flow cytometry analysis showed that WJ-MSCs were positive for MSC's markers CD73, CD90 and CD105 and negative for hematopoietic markers CD34 and CD45. Light microscopy showed development of nodule-like structures after 10 days of culture on (PAH-PSS)3-PAH, which resulted in a disturbance of cell monolayer. Whereas WJ-MSCs cultured on (PAH-PSS)4 ending with PSS showed a normal cell growth like on collagen and reached confluence after 10 days.The culture surface seems to have a determining role in WJ-MSC's "spatial" behavior, which could be considered in the field of tissue engineering. |
| Databáze: | OpenAIRE |
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