Alignment of Mitotic Chromosomes in Human Cells Involves SR-Like Splicing Factors Btf and TRAP150
| Autor: | Keshia Torres-Shafer, Vishnu Priya Battini, Athanasios Bubulya, Sapna N. Varia, Paula A. Bubulya, Divya Cheedu, Michael P. Markey |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
serine-arginine-rich (SR) proteins Btf TRAP150 pre-mRNA splicing mitosis cell cycle RNA Stability RNA-binding protein lcsh:Chemistry 0302 clinical medicine RNA Precursors RNA Small Interfering lcsh:QH301-705.5 Spectroscopy Serine-Arginine Splicing Factors Cell Cycle Nuclear Proteins RNA-Binding Proteins General Medicine Cell cycle Computer Science Applications DNA-Binding Proteins medicine.anatomical_structure Ribonucleoproteins RNA splicing Active Transport Cell Nucleus Biology DNA-binding protein Catalysis Chromosomes Article Inorganic Chemistry 03 medical and health sciences SR protein medicine Humans RNA Messenger Physical and Theoretical Chemistry Molecular Biology Mitosis Cell Nucleus Tumor Suppressor Proteins Organic Chemistry Molecular biology Repressor Proteins Cell nucleus 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Gene Expression Regulation Exon junction complex 030217 neurology & neurosurgery HeLa Cells Transcription Factors |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 18, Iss 9, p 1956 (2017) International Journal of Molecular Sciences; Volume 18; Issue 9; Pages: 1956 |
| ISSN: | 1422-0067 |
| Popis: | Serine-arginine-rich (SR) or SR-like splicing factors interact with exon junction complex proteins during pre-mRNA processing to promote mRNA packaging into mature messenger ribonucleoproteins (mRNPs) and to dictate mRNA stability, nuclear export, and translation. The SR protein family is complex, and while many classical SR proteins have well-defined mRNA processing functions, those of other SR-like proteins is unclear. Here, we show that depletion of the homologous non-classical serine-arginine-rich (SR) splicing factors Bcl2-associated transcription factor (Btf or BCLAF) and thyroid hormone receptor-associated protein of 150 kDa (TRAP150) causes mitotic defects. We hypothesized that the depletion of these SR-like factors affects mitosis indirectly through an altered expression of mitotic checkpoint regulator transcripts. We observed an altered abundance of transcripts that encode mitotic regulators and mitotic chromosome misalignment defects following Btf and/or TRAP150 depletion. We propose that, in addition to their previously reported roles in maintaining mRNA distribution, Btf and TRAP150 control the abundance of transcripts encoding mitotic regulators, thereby affecting mitotic progression in human cells. |
| Databáze: | OpenAIRE |
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