Retinoblastoma gene mutations in primary human prostate cancer
| Autor: | Yoshiko Iwasaki, Yasushi Dobashi, Kiyoshi Fujinami, Yoshinobu Kubota, Hitoshi Kitamura, Taro Shuin, Hiroji Uemura, Hiroshi Miyamoto |
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| Rok vydání: | 1995 |
| Předmět: |
Male
Urology Molecular Sequence Data Adenocarcinoma Biology Gene mutation Polymerase Chain Reaction law.invention Exon law medicine Humans Point Mutation Coding region RNA Neoplasm Genes Retinoblastoma Gene Polymorphism Single-Stranded Conformational Polymerase chain reaction DNA Primers Genetics Base Sequence Retinoblastoma Point mutation Prostate Prostatic Neoplasms Cancer DNA Neoplasm Exons medicine.disease Gene Expression Regulation Neoplastic Oncology Mutation Cancer research Gene Deletion |
| Zdroj: | The Prostate. 27:314-320 |
| ISSN: | 1097-0045 0270-4137 |
| DOI: | 10.1002/pros.2990270604 |
| Popis: | Structural alterations in the entire coding regions (exons 1 to 27) of the retinoblastoma (RB) gene in primary human prostate cancers were investigated, using polymerase chain reaction and single strand conformational polymorphism analysis of RNA. Of 25 samples obtained from patients, four (16.4%) were found to have RB alterations. DNA sequencing of the PCR products revealed point mutations resulting in single amino-acid substitutions of exons 6 and 19 in two cases, and base deletions of exons 8 and 17 in two cases. Two of four cases with RB mutations were moderately differentiated localized tumors and other two with RB mutations were poorly differentiated tumors with metastases. Our results suggest that RB gene mutation is involved in progression steps of prostate carcinogenesis. |
| Databáze: | OpenAIRE |
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