Surface Inoculation and Quantification of Pseudomonas syringae Population in the Arabidopsis Leaf Apoplast

Autor:	Shweta Panchal, Cristián Jacob, Maeli Melotto
Rok vydání:	2017
Předmět:	Foliar internalization 0106 biological sciences 0301 basic medicine Strategy and Management Population Pseudomonas syringae Biology 01 natural sciences Article Industrial and Manufacturing Engineering 03 medical and health sciences Arabidopsis Botany Leaf inoculation education education.field_of_study Inoculation Mechanical Engineering fungi Metals and Alloys food and beverages biology.organism_classification Plant tissue Apoplast Apoplastic bacterial population Emerging Infectious Diseases Infectious Diseases 030104 developmental biology Stomatal defense Infection 010606 plant biology & botany
Zdroj:	Bio-protocol, vol 7, iss 5
ISSN:	2331-8325
Popis:	Bacterial pathogens must enter the plant tissue in order to cause a successful infection. Foliar bacterial pathogens that are not able to directly penetrate the plant epidermis rely on wounds or natural openings to internalize leaves. This protocol describes a procedure to estimate the population size of Pseudomonas syringae in the leaf apoplast after surface inoculation of Arabidopsis rosettes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2d2484001b77325efd7bab448904e2f https://doi.org/10.21769/bioprotoc.2167 Zobrazit plný text záznamu