Sortase-Dependent Proteins Promote Gastrointestinal Colonization by Enterococci
| Autor: | Nita H. Salzman, Leou Ismael Banla, Adam M. Pickrum, Michael Hayward, Christopher J. Kristich |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Immunology Microbiology Enterococcus faecalis Mice Bacterial Proteins Sortase Cell Wall medicine Animals Humans Colonization biology Mucin Human gastrointestinal tract biology.organism_classification Aminoacyltransferases Host-Associated Microbial Communities Mucus Gastrointestinal Microbiome Gastrointestinal Tract Mice Inbred C57BL Cysteine Endopeptidases Disease Models Animal Infectious Diseases medicine.anatomical_structure Enterococcus Sortase A Parasitology |
| Zdroj: | Infection and immunity. 87(5) |
| ISSN: | 1098-5522 |
| Popis: | The human gastrointestinal tract (GIT) is inhabited by a dense microbial community of symbionts. Enterococci are among the earliest members of this community and remain core members of the GIT microbiota throughout life. Enterococci have also recently emerged as opportunistic pathogens and major causes of nosocomial infections. Although recognized as a prerequisite for infection, colonization of the GIT by enterococci remains poorly understood. One way that bacteria adapt to dynamic ecosystems like the GIT is through the use of their surface proteins to sense and interact with components of their immediate environment. In Gram-positive bacteria, a subset of surface proteins relies on an enzyme called sortase for covalent attachment to the cell wall. Here, we show that the housekeeping sortase A (SrtA) enzyme promotes intestinal colonization by enterococci. Furthermore, we show that the enzymatic activity of SrtA is key to the ability of Enterococcus faecalis to bind mucin (a major component of the GIT mucus). We also report the GIT colonization phenotypes of E. faecalis mutants lacking selected sortase-dependent proteins (SDPs). Further examination of the mucin binding ability of these mutants suggests that adhesion to mucin contributes to intestinal colonization by E. faecalis. |
| Databáze: | OpenAIRE |
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