A New Packing for Separation of DNA Restriction Fragments by High Performance Liquid Chromatography
| Autor: | Kenichi Matsubara, Yoshio Kato, Masaaki Sasaki, Tomoaki Murotsu, Tsutomu Hashimoto, Shinichi Fukushige |
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| Rok vydání: | 1984 |
| Předmět: |
Base Composition
Chromatography biology Base pair Analytical chemistry DNA DNA Restriction Enzymes General Medicine Fractionation Biochemistry High-performance liquid chromatography High pressure liquid chromatography procedure DNA restriction Restriction fragment chemistry.chemical_compound Chain length chemistry Chromatography Gel biology.protein Electrophoresis Polyacrylamide Gel Deoxyribonucleases Type II Site-Specific Molecular Biology Chromatography High Pressure Liquid |
| Zdroj: | The Journal of Biochemistry. 95:83-86 |
| ISSN: | 1756-2651 0021-924X |
| DOI: | 10.1093/oxfordjournals.jbchem.a134606 |
| Popis: | TSK-GEL SW was found to be useful as a packing in high performance liquid chromatography for the separation of double-stranded DNA restriction fragments. DNA fragments smaller than 300 base pairs were separated as discrete peaks depending solely upon difference in chain length. The recovery of DNA fragments was higher than 90%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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