Knockout of
| Autor: | Oksana Palchevska, Iga Wasilewska, Rishikesh Kumar Gupta, Jacek Kuźnicki |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
lcsh:Chemistry
Stim2a Gene Knockout Techniques lcsh:QH301-705.5 Zebrafish Spectroscopy Neurons biology Glutamate receptor Phototaxis STIM1 General Medicine STIM2 Computer Science Applications Cell biology Phenotype Larva calcium toolkit SOCE chemistry.chemical_element Glutamic Acid glutamate PTZ Calcium Hyperkinesis Catalysis Article Inorganic Chemistry Animals behavioral tests Calcium Signaling Physical and Theoretical Chemistry Stromal Interaction Molecule 2 Molecular Biology Thigmotaxis Sequence Analysis RNA Endoplasmic reticulum Gene Expression Profiling Organic Chemistry Wild type Zebrafish Proteins biology.organism_classification hyperactivity Disease Models Animal nervous system lcsh:Biology (General) lcsh:QD1-999 chemistry Pentylenetetrazole Transcription Factors |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 21, Iss 6198, p 6198 (2020) Volume 21 Issue 17 |
| ISSN: | 1422-0067 |
| Popis: | Stromal interaction molecule (STIM) proteins play a crucial role in store-operated calcium entry (SOCE) as endoplasmic reticulum Ca2+ sensors. In neurons, STIM2 was shown to have distinct functions from STIM1. However, its role in brain activity and behavior was not fully elucidated. The present study analyzed behavior in zebrafish (Danio rerio) that lacked stim2a. The mutant animals had no morphological abnormalities and were fertile. RNA-sequencing revealed alterations of the expression of transcription factor genes and several members of the calcium toolkit. Neuronal Ca2+ activity was measured in vivo in neurons that expressed the GCaMP5G sensor. Optic tectum neurons in stim2a&minus /&minus fish had more frequent Ca2+ signal oscillations compared with neurons in wildtype (WT) fish. We detected an increase in activity during the visual&ndash motor response test, an increase in thigmotaxis in the open field test, and the disruption of phototaxis in the dark/light preference test in stim2a&minus mutants compared with WT. Both groups of animals reacted to glutamate and pentylenetetrazol with an increase in activity during the visual&ndash motor response test, with no major differences between groups. Altogether, our results suggest that the hyperactive-like phenotype of stim2a&minus mutant zebrafish is caused by the dysregulation of Ca2+ homeostasis and signaling. |
| Databáze: | OpenAIRE |
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