The effect of light wavelength on in vitro bilirubin photodegradation and photoisomer production
Autor: | Benjamin K. Cline, Lucie Ludvíková, Lucie Muchová, Petr Klán, Hendrik J. Vreman, Libor Vítek, David K. Stevenson, Stephanie Kourula, Ronald J. Wong, Jana Jašprová |
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Rok vydání: | 2018 |
Předmět: |
Light
Bilirubin Serum albumin Quantum yield Serum Albumin Human In Vitro Techniques Photochemistry chemistry.chemical_compound Isomerism Spectrophotometry medicine Humans Photodegradation Action spectrum Photolysis medicine.diagnostic_test biology Lumirubin Infant Newborn Phototherapy Human serum albumin chemistry Pediatrics Perinatology and Child Health biology.protein Hyperbilirubinemia Neonatal medicine.drug |
Zdroj: | Pediatric research. 85(6) |
ISSN: | 1530-0447 |
Popis: | The action spectrum for bilirubin photodegradation has been intensively studied. However, questions still remain regarding which light wavelength most efficiently photodegrades bilirubin. In this study, we determined the in vitro effects of different irradiation wavelength ranges on bilirubin photodegradation. In our in vitro method, normalized absolute irradiance levels of 4.2 × 1015 photons/cm2/s from light-emitting diodes (ranging from 390–530 nm) and 10-nm band-pass filters were used to irradiate bilirubin solutions (25 mg/dL in 4% human serum albumin). Bilirubin and its major photoisomer concentrations were determined; the half-life time of bilirubin (t1/2) was calculated for each wavelength range, and the spectral characteristics for bilirubin photodegradation products were obtained for key wavelengths. The in vitro photodegradation of bilirubin at 37 °C decreased linearly as the wavelength was increased from 390 to 500 nm with t1/2 decreasing from 63 to 17 min, respectively. At 460 ± 10 nm, a significantly lower rate of photodegradation and thus higher t1/2 (31 min) than that at 500 nm (17 min) was demonstrated. In our system, the optimum bilirubin photodegradation and lumirubin production rates occurred between 490 and 500 nm. Spectra shapes were remarkably similar, suggesting that lumirubin production was the major process of bilirubin photodegradation. |
Databáze: | OpenAIRE |
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