Cardioprotection by minocycline in a rabbit model of ischemia/reperfusion injury: Detection of cell death by in vivo (111)In-GSAO SPECT
| Autor: | Hans J. de Haas, Atsuko Tahara, Jun Zhou, Dilbahar Mohar, H. William Strauss, Takayoshi Yamaki, Nobuhiro Tahara, Tiziano Scarabelli, Hendrikus H. Boersma, Annapoorna Kini, Neena B. Haider, Artiom Petrov, Jagat Narula, Yasuchika Takeishi |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Necrosis Ischemia PROTEIN Molecular imaging necrosis 03 medical and health sciences 0302 clinical medicine In vivo medicine Journal Article Radiology Nuclear Medicine and imaging acute coronary syndromes Myocardial infarction Cardioprotection apoptosis imaging business.industry Minocycline medicine.disease 030104 developmental biology ANNEXIN A5 MYOCARDIAL-INFARCTION 030220 oncology & carcinogenesis SPECT medicine.symptom Cardiology and Cardiovascular Medicine business Reperfusion injury Ex vivo medicine.drug |
| Zdroj: | Journal of Nuclear Cardiology, 25(1), 94-100. SPRINGER |
| ISSN: | 1071-3581 |
| Popis: | BACKGROUND: Preclinical studies indicate that minocycline protects against myocardial ischemia/reperfusion injury. In these studies, minocycline was administered before ischemia, which can rarely occur in clinical practice. The current study aimed to evaluate cardioprotection by minocycline treatment upon reperfusion.METHODS: Rabbits were subjected to myocardial ischemia/reperfusion injury and received either intravenous minocycline (n = 8) or saline (n = 8) upon reperfusion. Cardiac cell death was assessed by in vivo micro-SPECT/CT after injection of Indium-111-labeled 4-(N-(S-glutathionylacetyl)amino) phenylarsonous acid ((111)In-GSAO). Thereafter, hearts were explanted for ex vivo imaging, γ-counting, and histopathological characterization.RESULTS: Myocardial damage was visualized by micro-SPECT/CT imaging. Quantitative GSAO uptake (expressed as percent injected dose per gram, %ID/g) in the area at risk was lower in minocycline-treated animals than that in saline-treated control animals (0.32 ± 0.13% vs 0.48 ± 0.15%, P = 0.04). TUNEL staining confirmed the reduction of cell death in minocycline-treated animals.CONCLUSIONS: This study demonstrates cardioprotection by minocycline in a clinically translatable protocol. |
| Databáze: | OpenAIRE |
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