Ectopic expression of an apple apomixis-related gene MhFIE induces co-suppression and results in abnormal vegetative and reproductive development in tomato
| Autor: | Yu-Jin Hao, Ke-Qin Chen, Dan-Dan Liu, Mou-Jing Fang, Qing-Long Dong |
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| Rok vydání: | 2011 |
| Předmět: |
Physiology
Gene Expression Germination Plant Science Flowers Biology Parthenocarpy Real-Time Polymerase Chain Reaction Sepal Transformation Genetic Solanum lycopersicum Gene Expression Regulation Plant Two-Hybrid System Techniques Botany Arabidopsis thaliana Genetically modified tomato Ovule Plant Proteins Regulation of gene expression Reproduction fungi food and beverages biology.organism_classification Plants Genetically Modified Cell biology Plant Leaves Phenotype RNA Plant Fruit Malus Seeds Petal Ectopic expression RNA Interference Agronomy and Crop Science Apomixis |
| Zdroj: | Journal of plant physiology. 169(18) |
| ISSN: | 1618-1328 |
| Popis: | It has been well documented that FERTILIZATION-INDEPENDENT ENDOSPERM (FIE) plays important regulatory roles in diverse developmental processes in model plant Arabidopsis thaliana. However, it is largely unknown how FIE genes function in economically important crops. In this study, MhFIE gene, which was previously isolated from apomictic tea crabapple (Malus hupehensis Redh. var. pingyiensis), was introduced into tomato. The hemizygous transgenic tomato lines produced curly leaves and decreased in seed germination. In addition, the co-suppression of the transgenic MhFIE and endogenous (SlFIE) genes occurred in homozygous transgenic tomatoes. As a result, FIE silencing brought about abnormal phenotypes during reproductive development in tomato, such as increased sepal and petal numbers in flower, a fused ovule and pistil and parthenocarpic fruit formation. A yeast two-hybrid assay and bimolecular fluorescence complementation (BiFC) demonstrated that MhFIE interacted with a tomato protein, EZ2 (SlEZ2). Its ectopic expression and SlFIE co-suppression notably influenced the expression of genes associated with leaf, flower, and fruit development. Therefore, together with other PcG proteins, FIE was involved in the regulation of vegetative and reproductive development by modulating the expression of related genes in plants. |
| Databáze: | OpenAIRE |
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