Rapid aptasensor capable of simply diagnosing prostate cancer
| Autor: | Timothy Cha, Ji Hoon Lee, Sandy Cho, Young Teck Kim |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Guanine Aptamer Biomedical Engineering Biophysics Biosensing Techniques law.invention chemistry.chemical_compound law Limit of Detection Electrochemistry medicine Humans Chemiluminescence Fluorescent Dyes Detection limit medicine.diagnostic_test Prostatic Neoplasms General Medicine Aptamers Nucleotide Prostate-Specific Antigen Fluoresceins Prostate-specific antigen Biochemistry chemistry Energy Transfer Immunoassay Luminescent Measurements Biosensor DNA Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 62 |
| ISSN: | 1873-4235 |
| Popis: | Using guanine (G)-rich DNA aptamer-conjugated 6-carboxyfluorescein (6-FAM) capable of rapidly capturing prostate specific antigen (PSA) in human serum, cost-effective and simple biosensor with guanine chemiluminescence detection was developed for early diagnosis of prostate cancer. Free G-rich DNA aptamer-conjugated 6-FAM emits bright light in guanine chemiluminescence reaction based on the principle of chemiluminescent resonance energy transfer (CRET). However, G-rich DNA aptamer-conjugated 6-FAM bound with PSA cannot emit light because PSA acts as a strong interference in CRET between 6-FAM and high-energy intermediate formed from the reaction of 3,4,5-trimethoxylphenylglyoxal (TMPG) and guanine of G-rich DNA aptamer. A chemiluminescent biosensor, developed using the different properties of G-rich DNA aptamer-conjugated 6-FAM in the absence and presence of PSA in guanine chemiluminescence reaction, was able to quantify trace levels of PSA in human serum within 30 min without time-consuming and complicated procedures (e.g., multiple incubation and washings) required for conventional immunoassays operated with expensive and intractable antibodies. The limit of detection of chemiluminescent biosensor having a wide linear dynamic range (1.9–125 ng/ml) was 1.0 ng/ml. The excellent correlation ( R =0.985) between chemiluminescent biosensor and conventional enzyme immunoassay indicates that the accurate, precise, and rapid chemiluminescent biosensor can be applied as a new method for early diagnosis of prostate cancer. |
| Databáze: | OpenAIRE |
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