Combined pre- and postnatal ethanol exposure in rats disturbs the myelination of optic axons
| Autor: | Jose Javier Garcia-Medina, Vicente Vila-Bou, Maria D Pinazo-Duran, Pedro Sanz-Solana, Vicente Zanon-Moreno, C. Galbis-Estrada, Roberto Gallego-Pinazo, Sheila Pons-Vazquez |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Neurofilament Time Factors genetic structures Offspring Eye Retina Myelin chemistry.chemical_compound Neurofilament Proteins Pregnancy Internal medicine medicine Animals Axon Rats Wistar Myelin Sheath biology Ethanol Chemistry Body Weight Central Nervous System Depressants Retinal Myelin Basic Protein Optic Nerve General Medicine Anatomy eye diseases Axons Myelin basic protein Rats Disease Models Animal medicine.anatomical_structure Endocrinology nervous system Animals Newborn Prenatal Exposure Delayed Effects biology.protein Optic nerve Female |
| Zdroj: | Alcohol and alcoholism (Oxford, Oxfordshire). 46(5) |
| ISSN: | 1464-3502 |
| Popis: | Aims: To analyse myelination and outgrowth of the optic axons in relation to the neuro-ophthalmological manifestations of ethanol (EtOH) abuse during pregnancy. Methods: An experimental model of chronic EtOH exposure was developed in rats and their offspring by subjecting the dams to a liquid diet (35% of the daily total calories as either EtOH or maltose-dextrose nutritional controls (Con). Eyeballs and optic nerves were obtained at key developmental stages and processed for morphologic, immunocytochemical and immunoblotting procedures, using alternatively antibodies against myelin basic protein (MBP) or neurofilament (NF) protein, and image analysing. Results: A significant delay in onset of optic axons myelination, as well as a significant reduction in optic nerve size ( P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|