Molecular Cloning and Characterization of RGC-32, a Novel Gene Induced by Complement Activation in Oligodendrocytes
| Autor: | Florin Niculescu, Lucian Soane, Horea Rus, Tudor C. Badea, Moon L. Shin |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
DNA Replication DNA Complementary Molecular Sequence Data Muscle Proteins Cell Cycle Proteins Nerve Tissue Proteins Complement factor I Biochemistry Complement factor B Humans Amino Acid Sequence RNA Messenger Cloning Molecular Complement Activation Molecular Biology Decay-accelerating factor Base Sequence biology Complement component 2 CD46 Cell Biology Molecular biology Oligodendroglia Gene Expression Regulation Factor H biology.protein sense organs CFHR5 Complement control protein |
| Zdroj: | Journal of Biological Chemistry. 273:26977-26981 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.273.41.26977 |
| Popis: | Sublytic complement activation on oligodendrocytes (OLG) down-regulates expression of myelin genes and induces cell cycle in culture. Differential display (DD) was used to search for new genes whose expression is altered in response to complement and that may be involved in cell cycle activation. DD bands showing either increased or decreased mRNA expression in response to complement were identified and designated ResponseGenes to Complement (RGC) 1–32.RGC-1 is identical with heat shock protein 105,RGC-2 with poly(ADP-ribose) polymerase, andRGC-10 with IP-10. A new gene, RGC-32, that encodes a protein of 137 amino acids was cloned. RGC-32 has no homology with other known proteins, and contains no motif that would indicate its function. In OLG, the mRNA expression was increased by complement activation and by terminal complement complex assembly. RGC-32 protein was localized in the cytoplasm and co-immunoprecipitated with cdc2 kinase. Overexpression of RGC-32 increased DNA synthesis in OLGxC6 glioma cell hybrids. These results suggest thatRGC-32 may play a role in cell cycle activation. |
| Databáze: | OpenAIRE |
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