Can laboratory reference strains mirror ‘real-world’ pathogenesis?

Autor:	J. W. Costerton, Paul Stoodley, Christoph A Fux, Mark E. Shirtliff
Rok vydání:	2005
Předmět:	Microbiology (medical) Genetics Staphylococcus aureus Escherichia coli K12 Genetic Variation Bacterial genome size Biology Isolation (microbiology) Microbiology Genome Bacterial strain Pathogenesis Infectious Diseases Virology Pseudomonas aeruginosa Humans DNA microarray Genome Bacterial
Zdroj:	Trends in Microbiology. 13:58-63
ISSN:	0966-842X
DOI:	10.1016/j.tim.2004.11.001
Popis:	The extraordinary plasticity of bacterial genomes raises concerns about the adequacy of laboratory-adapted reference strains for the study of "real-world" pathogenesis. Some laboratory strains have been sub-cultured for decades since their first isolation and might have lost important pathophysiological characteristics. Evidence is presented that bacteria rapidly adapt to in vitro conditions. Genomic differences between laboratory reference strains and corresponding low-passage clinical isolates are reviewed. It appears that no bacterial strain can truly represent its species. For DNA microarray and proteomic studies, this limitation might be overcome by the summation of individual genomes to produce a species-specific virtual supragenome.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2c4713ee87d20807fd7ea91fee08b26 https://doi.org/10.1016/j.tim.2004.11.001 Zobrazit plný text záznamu Full Text from ScienceDirect