FOXC2-AS1 stabilizes FOXC2 mRNA via association with NSUN2 in gastric cancer cells
| Autor: | Jianfa Lv, Juntao Liu, Wenwei Huang, Zhengbin Huang, Jijun Yan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Methyltransferase Cell Line Cell Movement Stomach Neoplasms medicine Humans Neoplasm Invasiveness Molecular Targeted Therapy RNA Messenger Cell Proliferation Neoplasm Staging Gene knockdown Messenger RNA Oncogene biology Chemistry RNA Cancer Forkhead Transcription Factors Cell Biology Methyltransferases Oncogenes medicine.disease Gene Expression Regulation Neoplastic Survival Rate Cancer cell biology.protein Cancer research RNA Long Noncoding FOXC2 |
| Zdroj: | Human cell. 34(6) |
| ISSN: | 1749-0774 |
| Popis: | Long noncoding RNA (lncRNA) FOXC2-AS1 has been reported to act as an oncogene in multiple human cancers. However, the clinical significance, functional role and underlying mechanism of FOXC2-AS1 in gastric cancer (GC) remains largely unknown. Here, we found that FOXC2-AS1 expression was significantly elevated in GC tissues and cells, and overexpression of FOXC2-AS1 indicated advanced TNM stage and shorter overall survival in GC patients. Functionally, knockdown of FOXC2-AS1 attenuated the proliferation, migration and invasion of GC cells, whereas overexpression of FOXC2-AS1 showed the opposite effects. Further investigation revealed that FOXC2-AS1 interacted with FOXC2 mRNA and repressed its degradation. FOXC2-AS1 recruited RNA methyltransferase NSUN2 to FOXC2 mRNA, increasing its m5C level and association with YBX1. Taken together, our findings suggested that FOXC2-AS1 acted as an oncogenic lncRNA by stabilizing FOXC2 mRNA in an m5C-dependent manner, which may provide a novel therapeutic target for GC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink