The Shine-Dalgarno-like sequence is a negative regulatory element for translation of tobacco chloroplast rps2 mRNA: an additional mechanism for translational control in chloroplasts
| Autor: | Wojciech Plader, Masahiro Sugiura |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Ribosomal Proteins
Untranslated region Genetics Base Sequence RNA Chloroplast Five prime untranslated region Molecular Sequence Data Negative regulatory element Nucleic acid sequence Shine-Dalgarno sequence Translation (biology) Cell Biology Plant Science Biology Genetic translation Protein Biosynthesis Sequence Homology Nucleic Acid Tobacco Translational regulation RNA Messenger 5' Untranslated Regions Sequence Deletion |
| Zdroj: | The Plant Journal. 34:377-382 |
| ISSN: | 1365-313X 0960-7412 |
| DOI: | 10.1046/j.1365-313x.2003.01732.x |
| Popis: | Most prokaryotic mRNAs contain within the 5' untranslated region (UTR), a Shine-Dalgarno (SD) sequence, which is complementary to the 3' end of 16S rRNA and serves as a major determinant for correct translational initiation. The tobacco chloroplast rps2 mRNA possesses an SD-like sequence (GGAG) at a proper position (positions -8 to -5 from the start codon). Using an in vitro translation system from isolated tobacco chloroplasts, the role of this sequence in translation was examined. Unexpectedly, the mutation of the SD-like element resulted in a large increase in translation. Internal and external deletions within the 5' UTR revealed that the region from -20 to -5 was involved in the negative regulation of translation. Scanning mutagenesis assays confirmed the above result. Competition assays suggested the existence of a trans-acting factor(s) involved in translational regulation. In this study, we discuss a possible mechanism for the negative regulation of rps2 mRNA translation. |
| Databáze: | OpenAIRE |
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